Tricine
- CAS No.
- 5704-04-1
- Chemical Name:
- Tricine
- Synonyms
- TRIS-HCL BUFFER;1389475;TROMETHAMINE HYDROCHLORIDE;Ticine;Tricine buffer, free acid;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;TRICINE;TRIClNE;n-Tricine-D8;Tricine >99%
- CBNumber:
- CB5853856
- Molecular Formula:
- C6H13NO5
- Molecular Weight:
- 179.17
- MDL Number:
- MFCD00004277
- MOL File:
- 5704-04-1.mol
- MSDS File:
- SDS
| Melting point | 186-188 °C (dec.) |
|---|---|
| Boiling point | 311.69°C (rough estimate) |
| Density | 1.05 g/mL at 20 °C |
| bulk density | 500kg/m3 |
| vapor pressure | 0.002-0.004Pa at 20-25℃ |
| refractive index | 1.4240 (estimate) |
| storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
| solubility | H2O: 4 M at 20 °C, clear, colorless |
| form | crystalline |
| color | clear colorless (40 % (w/w) in H2O) solution |
| PH | 4.0-6.0 (1M in H2O) |
| pka | 8.1(at 25℃) |
| PH Range | 7.4 - 8.8 |
| Odor | Odorless |
| biological source | synthetic |
| Water Solubility | soluble |
| Merck | 14,9651 |
| BRN | 1937804 |
| Stability | Stable. |
| LogP | -2.87 at 25℃ |
| CAS DataBase Reference | 5704-04-1(CAS DataBase Reference) |
| FDA UNII | W12LH4V8V3 |
| EPA Substance Registry System | Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1) |
| Absorption |
≤0.05 at 280 in H2O at 1M ≤0.1 at 260 in H2O at 1M |
| UNSPSC Code | 12161705 |
| NACRES | ND.02 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H320 | |||||||||
| Precautionary statements | P403-P501c | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38 | |||||||||
| Safety Statements | 26-36-36/37/39 | |||||||||
| WGK Germany | 3 | |||||||||
| F | 3 | |||||||||
| TSCA | Yes | |||||||||
| HS Code | 29052900 | |||||||||
| NFPA 704 |
|
Tricine price More Price(79)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | T17917 | Tricine ≥99% (titration), reagent grade, crystalline | 5704-04-1 | 100G | $76.5 | 2024-03-01 | Buy |
| Sigma-Aldrich | T17917 | Tricine ≥99% (titration), reagent grade, crystalline | 5704-04-1 | 500G | $353 | 2024-03-01 | Buy |
| Sigma-Aldrich | T17917 | Tricine ≥99% (titration), reagent grade, crystalline | 5704-04-1 | 1KG | $483 | 2024-03-01 | Buy |
| Sigma-Aldrich | T17917 | Tricine ≥99% (titration), reagent grade, crystalline | 5704-04-1 | 5KG | $2000 | 2024-03-01 | Buy |
| Sigma-Aldrich | T17917 | Tricine ≥99% (titration), reagent grade, crystalline | 5704-04-1 | 10KG | $3770 | 2024-03-01 | Buy |
Tricine Chemical Properties,Uses,Production
Descriptioin
Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.3
Reference
- S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
- H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379
Chemical Properties
White/clear crystalline powder
Uses
Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).
Definition
ChEBI: N-tris(hydroxymethyl)methylglycine is a Good's buffer substance, pKa = 8.15 at 20 ℃. It is functionally related to a member of tris and a glycine. It is a tautomer of a N-tris(hydroxymethyl)methylammonioacetate.
General Description
Tricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.
Biological Activity
Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.
Purification Methods
Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]
Tricine Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Wuhan Fortuna Chemical Co., Ltd | +86-027-59207850 | info@fortunachem.com | China | 5969 | 58 |
| Suzhou Genemill Biotechnology Co.,Ltd | +8615862502491 | aimeegao@genemill.com | China | 39 | 58 |
| Hebei Mujin Biotechnology Co.,Ltd | +86 13288715578 +8613288715578 | sales@hbmojin.com | China | 12814 | 58 |
| Hebei Yanxi Chemical Co., Ltd. | +8617531153977 | allison@yan-xi.com | China | 5854 | 58 |
| Hebei Chuanghai Biotechnology Co,.LTD | +86-13131129325 | sales1@chuanghaibio.com | China | 5870 | 58 |
| Hebei Chuanghai Biotechnology Co., Ltd | +8615531151365 | mina@chuanghaibio.com | China | 18137 | 58 |
| Apeloa production Co.,Limited | +8619933239880 | admin@apcl.com.cn | China | 861 | 58 |
| Shanghai Daken Advanced Materials Co.,Ltd | +86-371-+86-371-66670886 | info@dakenam.com | China | 19777 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21629 | 55 |
| Neostar United (Changzhou) Industrial Co., Ltd. | +86-0519-85551759 +8613506123987 | marketing1@neostarunited.com | China | 8831 | 58 |
Related Qustion
- Q:How to prepare Tricine buffer
- A:Tricine is a commonly used electrophoresis buffer for resuspension of cell pellets. It has a lower negative charge than glycin....
- Jul 22，2024
View Lastest Price from Tricine manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2025-04-16 | Tricine
5704-04-1
|
US $0.00-0.00 / Kg/Drum | 1KG | 99%-101% | 5 tons | WUHAN FORTUNA CHEMICAL CO., LTD | |
 |
2025-04-16 | Tricine
5704-04-1
|
US $0.00-0.00 / kg | 1kg | 98 | 1000 | Wuhan Circle Star Chem-medical Technology Co.,Ltd | |
 |
2025-04-15 | Tricine
5704-04-1
|
US $30.00-10.00 / KG | 50KG | 99% | 500000kg | Hebei Chuanghai Biotechnology Co., Ltd |