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Perspectives of biotechnological production of l-tyrosine and its applications

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Abstract

The aromatic amino acid l-tyrosine is used as a dietary supplement and has promise as a valuable precursor compound for various industrial and pharmaceutical applications. In contrast to chemical production, biotechnological methods can produce l-tyrosine from biomass feedstocks under environmentally friendly and near carbon-free conditions. In this minireview, various strategies for synthesizing l-tyrosine by employing biocatalysts are discussed, including initial approaches as well as more recent advances. Whereas early attempts to engineer l-tyrosine-excreting microbes were based on auxotrophic and antimetabolite-resistant mutants, recombinant deoxyribonucleic acid technology and a vastly increasing knowledge of bacterial physiology allowed recently for more targeted genetic manipulations and strain improvements. As an alternative route, l-tyrosine can also be obtained from the conversion of phenol, pyruvate, and ammonia or phenol and serine in reactions catalyzed by the enzyme tyrosine phenol lyase.

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Acknowledgement

Financial support from the Singapore–MIT Alliance and fellowships from the Deutsche Forschungsgemeinschaft (TLE) and the National Science Foundation (CNS) are gratefully acknowledged.

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  1. Tina Lütke-Eversloh

    Present address: Institut für Biowissenschaften, Mikrobiologie, Universität Rostock, Albert-Einstein-Str.3, Rostock, 18059, Germany

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  1. Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA, 02139, USA

    Tina Lütke-Eversloh, Christine Nicole S. Santos & Gregory Stephanopoulos

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Lütke-Eversloh, T., Santos, C.N.S. & Stephanopoulos, G. Perspectives of biotechnological production of l-tyrosine and its applications. Appl Microbiol Biotechnol 77, 751–762 (2007). https://doi.org/10.1007/s00253-007-1243-y

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