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Structure of the human ADP-ribosylation factor 1 complexed with GDP

Nature volume 372, pages 704–708 (1994)Cite this article

Abstract

ADP-ribosylation factors (ARFs) are essential and ubiquitous in eukaryotes, being involved in vesicular transport and functioning as an activator of phospholipase D (refs 1,2) and cholera toxin3,4. The functions of ARF proteins in membrane traffic and organelle integrity5,6 are intimately tied to its reversible association with membranes and specific interactions with membrane phospholipids. One common feature of these functions is their regulation by the binding and hydrolysis of GTP. Here we report the three-dimensional structure of full-length human ARF1 (Mr 21,000) in its GDP-bound non-myristoylated form. The presence of a unique amino-terminal α-helix and loop, together with differences in Mg2+ ligation and the existence of a non-crystallographic dimer, set this structure apart from other GTP-binding proteins. These features provide a structural basis for the GTP-dependent modulation of membrane affinity, the lack of intrinsic GTPase activity, and the nature of effector binding surfaces.

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Authors and Affiliations

  1. Department of Biochemistry, Program in Bioorganic Chemistry, Brandeis University, Waltham, Massachusetts, 02254, USA

    Juan Carlos Amor

  2. Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts, 02254, USA

    David H. Harrison & Dagmar Ringe

  3. Department of Biochemistry and Chemistry, Brandeis University, Waltham, Massachusetts, 02254, USA

    Dagmar Ringe

  4. Laboratory of Biological Chemistry, Developmental Therapeutics Program, National Cancer Institute, Bethesda, Maryland, 20892, USA

    Richard A. Kahn

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  1. Juan Carlos Amor
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  4. Dagmar Ringe
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Carlos Amor, J., Harrison, D., Kahn, R. et al. Structure of the human ADP-ribosylation factor 1 complexed with GDP. Nature 372, 704–708 (1994). https://doi.org/10.1038/372704a0

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