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  • Oncogenomics
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Cloning and characterization of a novel 90 kDa ‘companion’ auto-antigen of p62 overexpressed in cancer

Oncogene volume 21, pages 5006–5015 (2002)Cite this article

Abstract

Recently our laboratory identified a cytoplasmic RNA-binding protein p62 which binds to and regulates the expression of IGF II mRNA. p62 was initially shown to be recognized by auto-antibodies in hepatocellular carcinoma (HCC) but now anti-p62 has been described in diverse malignancies. p62 is uniformly expressed in fetal liver and prominently in 33% of HCC nodules, but not detectable in adult liver or normal tissue adjacent to HCC nodules. In this study, a 90 kDa protein (p90), auto-antibodies to which were found associated with anti-p62 responses in the same HCC patient group, was identified by cDNA expression cloning. Indirect immunofluorescence showed that, like p62, p90 localized to the cytoplasm in cultured cells and mouse fetal, but not adult liver. Among 11 human gastric cancer tissues examined, p90 was overexpressed in six (55%). Together with other cancer associated auto-antibodies such as anti-p53, anti-p62, anti-Koc, and anti-CENP-F, auto-antibodies to p90 represent a new marker for tumors such as HCC and gastric cancer. Our data support the working hypothesis that auto-antibody production in cancer may be directly linked to aberrant auto-antigen expression.

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Abbreviations

GC:

gastric cancer

HCC:

hepatocellular carcinoma

IMP:

IGF-II mRNA binding protein

IP:

immunoprecipitation

TnT:

in vitro transcription and translation

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Acknowledgements

We thank Dr Eng M Tan for his encouragement, support and comments during this study. This is publication 14396-MEM from The Scripps Research Institute. This work was supported in part by National Institutes of Health Grants CA56956 and AI39645, the Sam and Rose Stein Charitable Trust, and NIH grant M01RR00833 provided to the General Clinical Research Center of The Scripps Research Institute.

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  1. Jianying Y Zhang

    Present address: Department of Biological Sciences, University of Texas at El Paso, 500 West University Avenue, El Paso, 79968-0519, TX

Authors and Affiliations

  1. Department of Molecular and Experimental Medicine, WM Keck Autoimmune Disease Center and DNA Core Laboratory for Structural Analysis, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, 92037, California, CA, USA

    Linda Soo Hoo, Jianying Y Zhang & Edward KL Chan

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  1. Linda Soo Hoo
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  2. Jianying Y Zhang
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Correspondence to Edward KL Chan.

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Hoo, L., Zhang, J. & Chan, E. Cloning and characterization of a novel 90 kDa ‘companion’ auto-antigen of p62 overexpressed in cancer. Oncogene 21, 5006–5015 (2002). https://doi.org/10.1038/sj.onc.1205625

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