Recipe

LB agar

Agar (20 g/L)

NaCl (10 g/L; Sigma-Aldrich S9625)

Tryptone (10 g/L; BD 211705)

Yeast extract (5 g/L; BD 212750)

Add H2O to a final volume of 1 L. Adjust the pH to 7.0 with 5 N NaOH. Autoclave. Pour into Petri dishes (~25 mL per 100-mm plate).

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  1. doi:10.1101/pdb.rec11683 Cold Spring Harb Protoc 2009: pdb.rec11683-

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