Biochemical and Biological Studies on Dopastin, an Inhibitor of Dopamine β-Hydroxylase

Hironobu IINUMA; Meiki MATSUZAKI; Toshiharu NAGATSU; Tomio TAKEUCHI; Hamao UMEZAWA

doi:10.1271/bbb1961.38.2107

Agricultural and Biological Chemistry

Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369

Biochemical and Biological Studies on Dopastin, an Inhibitor of Dopamine β-Hydroxylase

Hironobu IINUMA, Meiki MATSUZAKI, Toshiharu NAGATSU, Tomio TAKEUCHI, Hamao UMEZAWA

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1974 Volume 38 Issue 11 Pages 2107-2111

DOI https://doi.org/10.1271/bbb1961.38.2107

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Abstract

Dopastin produced by a pseudomonas is a potent inhibitor of dopamine β-hydroxylase. Kinetic studies with the purified enzyme indicated that inhibition by dopastin was of the uncompetitive type to the substrate and of the competitive to the cofactor, ascorbic acid. The nitrosohydroxylamino group of dopastin was found to be essential in inhibition of dopamine β-hydroxylase. Both racemic and natural dopastins showed the same activity. Dopastin showed significant hypotensive effect to spontaneously hypertensive rats and phytotoxicity to barley germination.

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