Skip to main content
Springer Nature Link
Log in

Degradation of polyethylene succinate (PES) by a new thermophilic Microbispora strain

  • Original Paper
  • Published:
Biodegradation Aims and scope Submit manuscript

Abstract

Thermophilic actinomycetes were isolated from sediment of the Chingshuei hot spring in north Taiwan, and the strain HS 45-1 was selected from colonies which formed distinct clear zones on agar plate with emulsified polyethylene succinate (PES). The film of PES disappeared within 6 days in liquid cultures at 50°C. The strain HS 45-1 was also able to degrade poly (ε-carpolactone) (PCL) and poly (3-hydroxybutyrate) (PHB) films completely within 6 days in liquid cultures. Basing on the results of phynotypic characteristics, phylogenetic studies and DNA-DNA hybridization, strain HS 45-1 should be assigned to Micorbispora rosea subsp. taiwanensis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

PES:

polyethylene succinate

PCL:

poly (ε-carpolactone)

PHB:

poly (3-hydroxybutyrate)

References

  • Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1997) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    Google Scholar 

  • Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Felsenstein J (1993) PHYLIP (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA

    Google Scholar 

  • Fields RD, Rodrigue F, Finn RK (1974) Microbial degradation of polyesters: Polycaprolactone degraded by P. pullulans. J Appl Polym Science 18:3571–3576

    Google Scholar 

  • Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322

    CAS  Google Scholar 

  • Hayakawa M, Nonomura H (1987) Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509

    Article  CAS  Google Scholar 

  • Ikura Y, Kudo T (1999) Isolation of a microorganism capable of degrading poly-(L-lactide). J Gen Appl Microbiol 45:247–251

    Article  CAS  Google Scholar 

  • Jarerat A, Pranamuda H, Tokiwa Y (2002) Poly (L-lactide)-Degrading Activity in Various Actinomycetes, Macromol. Biosci 2:420–428

    CAS  Google Scholar 

  • Jarerat A, Tokiwa Y (2003) Poly (L-lactide) degradation by Saccharothrix wayandensis. Biotechnol Lett 25:401–404

    Article  CAS  Google Scholar 

  • Jarerat A, Tokiwa Y (2003) Poly (L-lactide) degradation by Kibdelosporangium aridum. Biotechnol Lett 25:2035–2038

    Article  CAS  Google Scholar 

  • Kawamoto I, Oka T, Nara T (1981) Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. J Bacteriol 146:527–534

    CAS  Google Scholar 

  • Kelly KL (1966) Inter-Society Color Council–National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. US Government Printing Office, Washington, DC

    Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120

    Article  CAS  Google Scholar 

  • Kimura M (1983) The neutral theory of molecular evolution. Cambridge University Press, Cambridge

    Google Scholar 

  • Kohei N, Toshio T, Naoki A, Yoshiyuki K (2001) Purification and Characterization of an Extracellular Poly(L-Lactic Acid) Depolymerase from a Soil Isolate, Amycolatopsis sp. Strain K104–1. J Gen Appl Microbiol 67:345–353

    Article  Google Scholar 

  • Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17:1244–1245

    Article  CAS  Google Scholar 

  • Minnikin DE, Alshamaony L, Goodfellow M (1975) Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of␣whole-cell methanolysates. J Gen Microbiol 88:200–204

    CAS  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Nakajima Y, Kitpreechavanich V, Suzuki K, Kudo T (1999) Microbispora corallina sp. nov. a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767

    Google Scholar 

  • Nishida H, Tokiwa Y (1993) Distribution of poly (3-hydroxy-Butyrate) and poly (ε-caprolactone) aerobic degrading microorganism in different environments. J␣Environ Polym Degrad 1:227–233

    Article  CAS  Google Scholar 

  • Pranamuda H, Tokiwa Y, Tanaka H (1995) Microbial degradation of an aliphatic polyester with a high melting point poly (tetramehtylene succinate). Appl Environ Microbiol 61:1828–1832

    Google Scholar 

  • Pranamuda H, Tokiwa Y, Tanaka H (1997) Polylactide Degradation by an Amycolatopsis sp. J Gen Appl Microbiol 63:1637–1640

    CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Tansengco ML, Tokiwa Y (1998) Thermophilic microbial degradation of poly(ethylene succinate). World J Microbiol & Biotechnol 14:133–138

    Article  CAS  Google Scholar 

  • Tansengco ML, Dogma IJ Jr (1998) Comparative population study of aliphatic polyesters-degrading microorganisms at 50 °C. Chem Lett 1043–1044

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Tokiwa Y, Iwamoto A, Koyama M, Kataka N, Nishida H (1992) Biological recycling of plastics containing ester bonds. Makromolekulare Chemic-Makromolecular Symposia 57:273–279

    CAS  Google Scholar 

  • Tokiwa Y, Pranamuda H (2001) Microbial degradation of aliphatic polyester. In: Doi Y, Steinbuchel A (eds) Biopolymers, Vol 3, Weinheimi Wiley-VCH pp 85–103

  • Sanchez JG, Tsuchii A, Tokiwa Y (2000) Degradation of polycaprolactone at 50°C by a thermotolerant Aspergillus sp. Biotechnol Lett 22:849–853

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work is supported by the Ministry of Economic Affairs, R. O. C. (project no. 93-EC-17-A-17-R7-0525). We also thank Mr. Y. K. Lin for assistance in samples collection.

Author information

Authors and Affiliations

  1. Department of Chemical and Material Engineering, Ta-Hwa Institute of Technology, 1, Ta-Hwa Road, Chiung-Lin, Hsinchu, 30743, Taiwan, ROC

    Kim-Chi Hoang & Wei-Jye Shu

  2. Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331, Shih-Pin Road, Hsinchu, 300, Taiwan, ROC

    Min Tseng

Authors
  1. Kim-Chi Hoang
    View author publications

    You can also search for this author inPubMed Google Scholar

  2. Min Tseng
    View author publications

    You can also search for this author inPubMed Google Scholar

  3. Wei-Jye Shu
    View author publications

    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence to Wei-Jye Shu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hoang, KC., Tseng, M. & Shu, WJ. Degradation of polyethylene succinate (PES) by a new thermophilic Microbispora strain. Biodegradation 18, 333–342 (2007). https://doi.org/10.1007/s10532-006-9067-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10532-006-9067-5

Keywords