CN104041547A - Natural bactericide for controlling strawberry leaf spot and preparation method thereof - Google Patents
Natural bactericide for controlling strawberry leaf spot and preparation method thereof Download PDFInfo
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- CN104041547A CN104041547A CN201410236399.3A CN201410236399A CN104041547A CN 104041547 A CN104041547 A CN 104041547A CN 201410236399 A CN201410236399 A CN 201410236399A CN 104041547 A CN104041547 A CN 104041547A
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Abstract
A natural bactericide for controlling strawberry leaf spot is prepared from the following compositions: artemisia argyi, stemona sessilifolia, rhubarb, cortex phellodendri, gleditsia sinensis, dandelion, asarum herb, ginkgo biloba, croton seed and rhizoma cyrtomii fortunei. The natural bactericide for controlling strawberry leaf spot is abundant in raw-material source and low is cost, is prepared by employing pure natural compositions, is non-toxic and free of public hazard, and also is capable of effectively controlling strawberry leaf spot.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent of preventing and treating white leaf spot of strawberry and preparation method thereof.
Background technology
White leaf spot of strawberry claims again purple spot, focal spot disease, and China occurs commonplace, is one of strawberry leaves portion Common Diseases, harm occurs sometimes quite serious.White leaf spot of strawberry cause of disease is pineapple strawberry brown patch germ Marssonina potentillae (Desmazieres) Magn, and the perfect stage is that Diplocarpon earliana (Ellet EV.) belongs to Ascomycotina.Germ, discharges ascospore or conidium spring and borrows air to spread propagation, infect morbidity in plant diseased tissues or land on invalid thing and survive the winter with perithecium or pycnidium, and in seedling carries out in spite of illness, long-distance communications.This disease belongs to low temperature properties disease, and autumn and the more weather of rain and dew in early spring are conducive to infect morbidity.The strong morbidity of fertile sufficient seedling is light, and the weak morbidity of fertilizer deficiency seedling is heavy.Between strawberry cultivars, disease resistance is variant, and new star, to reach the kinds such as Na more disease-resistant.
White leaf spot of strawberry mainly, in morbidity in season in spring and autumn, is encroached on blade, petiole, carpopodium and calyx.On blade, produce the matt fleck of puce, be expanded into the irregular shape scab of 3-4 millimeters of diameters later, scab central authorities are little with periphery change color, scab has the tendency distributing along vein, when morbidity is heavy, blade face is covered with scab, and the later stage, full leaf was yellowish-brown to crineous, until withered.In the withered part of scab, grow black small grain point, after petiole or carpopodium morbidity, produce the scab that pitchy slightly caves in, sick portion tissue becomes fragile and frangibility.
At present, in vegetables, melon and fruit plantation, for preventing and treating white leaf spot of strawberry normally used, be chemical pesticide bactericide, because a large amount of problem of environmental pollutions that use chemical pesticide to cause are on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classifications produce drug resistances, pesticide resistance, increased the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, to useful biology, can threaten yet.And a lot of chemical pesticides are non-degradables or are difficult to degraded, easily pollute the enrichment of thing at animals and plants cylinder accumulation.
Along with the public is more and more higher to the requirement of " pollution-free food ", " pollution-free food ", also increase gradually to ecological agricultural chemical demand pollution-free, can natural degradation in market.From the development of pesticide science, on pesticide manufacturing is used, " Harmless " of " back to nature " and agricultural chemicals, be the inexorable trend of society and Development of Natural Science, so new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control white leaf spot of strawberry that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent of preventing and treating white leaf spot of strawberry provided by the invention, by following component, made: tarragon, the tuber of stemona, rheum officinale, golden cypress, Chinese honey locust, dandelion, the root of Chinese wild ginger, ginkgo leaf, crotons, rhizome of cyrtomium.
Preferably, by the component of following weight portion, made: tarragon 5-15 part, tuber of stemona 5-15 part, rheum officinale 8-12 part, golden cypress 8-12 part, Chinese honey locust 4-6 part, dandelion 4-6 part, root of Chinese wild ginger 4-6 part, ginkgo leaf 2-4 part, crotons 1-3 part, rhizome of cyrtomium 1-3 part.
More preferably, by the component of following weight portion, made: 10 parts of tarragons, 10 parts of the tubers of stemona, 10 parts of rheum officinales, 10 parts of golden cypresses, 5 parts of Chinese honey locusts, 5 parts of dandelions, 5 parts of the roots of Chinese wild ginger, 3 parts of ginkgo leaves, 2 parts of crotons, 2 parts of rhizome of cyrtomium.
The present invention also provides the preparation method of the natural bactericidal agent of above-mentioned control white leaf spot of strawberry, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is tarragon, the tuber of stemona, rheum officinale, golden cypress, Chinese honey locust, dandelion, the root of Chinese wild ginger, ginkgo leaf, crotons and rhizome of cyrtomium;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-70 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-70 ℃ and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of white leaf spot of strawberry.
The present invention also provides the application of the natural bactericidal agent of above-mentioned control white leaf spot of strawberry, is specially: the natural bactericidal agent of control white leaf spot of strawberry is mixed and is sterilization microbial inoculum with 500-1000 times of water gaging.
Beneficial effect: the natural bactericidal agent raw material sources of control white leaf spot of strawberry provided by the invention are abundant, with low cost, adopts the component of pure natural to make, Nonpoisonous, non-environmental-pollution not only, and can effectively prevent and treat white leaf spot of strawberry.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described concrete material proportion of embodiment, process conditions and result thereof be only for the present invention is described, and should also can not limit the present invention described in detail in claims.
Embodiment 1
The natural bactericidal agent of control white leaf spot of strawberry, is made by the component of following weight portion: 10 parts of tarragons, 10 parts of the tubers of stemona, 10 parts of rheum officinales, 10 parts of golden cypresses, 5 parts of Chinese honey locusts, 5 parts of dandelions, 5 parts of the roots of Chinese wild ginger, 3 parts of ginkgo leaves, 2 parts of crotons, 2 parts of rhizome of cyrtomium.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is tarragon, the tuber of stemona, rheum officinale, golden cypress, Chinese honey locust, dandelion, the root of Chinese wild ginger, ginkgo leaf, crotons and rhizome of cyrtomium;
(2) compound is added to the ethanol water of 8 times of amounts 95%, be heated to 60 ℃ and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times of amounts 95%, is heated to 60 ℃ and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/15 of total amount, adds the water of 2 times of volumes of concentrate, standing 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of white leaf spot of strawberry.
Embodiment 2
The natural bactericidal agent of control white leaf spot of strawberry, is made by the component of following weight portion: 5 parts of tarragons, 15 parts of the tubers of stemona, 8 parts of rheum officinales, 12 parts of golden cypresses, 4 parts of Chinese honey locusts, 6 parts of dandelions, 4 parts of the roots of Chinese wild ginger, 4 parts of ginkgo leaves, 3 parts of crotons, 1 part of rhizome of cyrtomium.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is tarragon, the tuber of stemona, rheum officinale, golden cypress, Chinese honey locust, dandelion, the root of Chinese wild ginger, ginkgo leaf, crotons and rhizome of cyrtomium;
(2) compound is added to the ethanol water of 5 times of amounts 95%, be heated to 70 ℃ and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times of amounts 95%, is heated to 70 ℃ and decocts 4h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/20 of total amount, adds the water of 1 times of volume of concentrate, standing 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of white leaf spot of strawberry.
Embodiment 3
The natural bactericidal agent of control white leaf spot of strawberry, is made by the component of following weight portion: 15 parts of tarragons, 5 parts of the tubers of stemona, 12 parts of rheum officinales, 8 parts of golden cypresses, 6 parts of Chinese honey locusts, 4 parts of dandelions, 6 parts of the roots of Chinese wild ginger, 2 parts of ginkgo leaves, 1 part of croton, 3 parts of rhizome of cyrtomium.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is tarragon, the tuber of stemona, rheum officinale, golden cypress, Chinese honey locust, dandelion, the root of Chinese wild ginger, ginkgo leaf, crotons and rhizome of cyrtomium;
(2) compound is added to the ethanol water of 10 times of amounts 95%, be heated to 50 ℃ and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times of amounts 95%, is heated to 50 ℃ and decocts 2h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/10 of total amount, adds the water of 3 times of volumes of concentrate, standing 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of white leaf spot of strawberry.
The natural bactericidal agent of the control white leaf spot of strawberry of embodiment 4 mensuration embodiment 1 to 3 is active to the inhibition of strawberry pathogen
This experiment is in vitro, and the natural bactericidal agent of the control white leaf spot of strawberry of mensuration embodiment 1 to 3 is active to the inhibition of strawberry pathogen, and method and result are as follows:
1. for examination bacterial classification
Du Lazhu is commercially available every spore Ramularia tulasnei (R.fragariae Peck).
2. Toxicity Determination
Utilize mycelial growth rate method and spore germination method measure the embodiment of the present invention 1 natural bactericidal agent to Du Lazhu the virulence every spore Ramularia tulasnei (R.fragariae Peck), by following formula calculating inhibiting rate:
3. experimental result
With growth rate method, measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for stipulating in < < pesticide field efficacy medicine test criterion > >, field efficacy to the natural bactericidal agent of the embodiment of the present invention 1 to 3 is tested, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
The natural bactericidal agent treatment white leaf spot of strawberry that adopts embodiment 1 to 3, various diseases is respectively treated 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment white leaf spot of strawberry of table 3 embodiment 1 to 3
Claims (5)
1. a natural bactericidal agent of preventing and treating white leaf spot of strawberry, is characterized in that: by following component, made: tarragon, the tuber of stemona, rheum officinale, golden cypress, Chinese honey locust, dandelion, the root of Chinese wild ginger, ginkgo leaf, crotons, rhizome of cyrtomium.
2. a kind of natural bactericidal agent of preventing and treating white leaf spot of strawberry according to claim 1, is characterized in that: the component by following weight portion is made: tarragon 5-15 part, tuber of stemona 5-15 part, rheum officinale 8-12 part, golden cypress 8-12 part, Chinese honey locust 4-6 part, dandelion 4-6 part, root of Chinese wild ginger 4-6 part, ginkgo leaf 2-4 part, crotons 1-3 part, rhizome of cyrtomium 1-3 part.
3. a kind of natural bactericidal agent of preventing and treating white leaf spot of strawberry according to claim 1, is characterized in that: the component by following weight portion is made: 10 parts of tarragons, 10 parts of the tubers of stemona, 10 parts of rheum officinales, 10 parts of golden cypresses, 5 parts of Chinese honey locusts, 5 parts of dandelions, 5 parts of the roots of Chinese wild ginger, 3 parts of ginkgo leaves, 2 parts of crotons, 2 parts of rhizome of cyrtomium.
4. a preparation method who prevents and treats the natural bactericidal agent of white leaf spot of strawberry, is characterized in that: comprise the following steps:
Raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is tarragon, the tuber of stemona, rheum officinale, golden cypress, Chinese honey locust, dandelion, the root of Chinese wild ginger, ginkgo leaf, crotons and rhizome of cyrtomium;
Add 5-10 doubly to measure 95% ethanol water compound, be heated to 50-70 ℃ and decoct 2-4h;
Filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-70 ℃ and decocts 2-4h;
Filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
Filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of white leaf spot of strawberry.
5. the application of the natural bactericidal agent of control white leaf spot of strawberry in control white leaf spot of strawberry, is characterized in that: the natural bactericidal agent of control white leaf spot of strawberry is mixed and is sterilization microbial inoculum with 500-1000 times of water gaging.
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Cited By (7)
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| CN104982263A (en) * | 2015-06-26 | 2015-10-21 | 安徽禾众农业科技有限公司 | Prevention and treatment method for white leaf spot of strawberries |
| CN105010429A (en) * | 2015-06-26 | 2015-11-04 | 安徽禾众农业科技有限公司 | Strawberry white leaf spot prevention and control bacteriostat, and preparation method and application thereof |
| CN107079931A (en) * | 2017-05-03 | 2017-08-22 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating reed wormwood artemisia virosis |
| CN107079932A (en) * | 2017-05-03 | 2017-08-22 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia virosis and its application |
| CN107711911A (en) * | 2017-11-09 | 2018-02-23 | 淮南市永光草莓专业合作社 | For preventing and treating the composition of white leaf spot of strawberry |
| CN108077253A (en) * | 2018-01-16 | 2018-05-29 | 浙江大学 | Application of the C6 volatile Aldehyde components in strawberry spherical cavity bacterium is inhibited |
| CN108371187A (en) * | 2018-01-16 | 2018-08-07 | 浙江大学 | Application of the terpene volatile materials in inhibiting strawberry spherical cavity bacterium |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982263A (en) * | 2015-06-26 | 2015-10-21 | 安徽禾众农业科技有限公司 | Prevention and treatment method for white leaf spot of strawberries |
| CN105010429A (en) * | 2015-06-26 | 2015-11-04 | 安徽禾众农业科技有限公司 | Strawberry white leaf spot prevention and control bacteriostat, and preparation method and application thereof |
| CN107079931A (en) * | 2017-05-03 | 2017-08-22 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating reed wormwood artemisia virosis |
| CN107079932A (en) * | 2017-05-03 | 2017-08-22 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia virosis and its application |
| CN107711911A (en) * | 2017-11-09 | 2018-02-23 | 淮南市永光草莓专业合作社 | For preventing and treating the composition of white leaf spot of strawberry |
| CN108077253A (en) * | 2018-01-16 | 2018-05-29 | 浙江大学 | Application of the C6 volatile Aldehyde components in strawberry spherical cavity bacterium is inhibited |
| CN108371187A (en) * | 2018-01-16 | 2018-08-07 | 浙江大学 | Application of the terpene volatile materials in inhibiting strawberry spherical cavity bacterium |
| CN108371187B (en) * | 2018-01-16 | 2019-09-10 | 浙江大学 | Terpene volatile materials is inhibiting the application in strawberry spherical cavity bacterium |
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| CN104041547B (en) | 2015-11-18 |
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