CN107372633B - Application of Artemisia annua tobacco vinegar to inhibit root-knot nematode - Google Patents

Application of Artemisia annua tobacco vinegar to inhibit root-knot nematode Download PDF

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CN107372633B
CN107372633B CN201710689170.9A CN201710689170A CN107372633B CN 107372633 B CN107372633 B CN 107372633B CN 201710689170 A CN201710689170 A CN 201710689170A CN 107372633 B CN107372633 B CN 107372633B
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artemisia annua
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CN107372633A (en
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李维蛟
徐福荣
浦仕彪
古今
马晓惠
李文春
董鲜
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Yunnan University of Traditional Chinese Medicine TCM
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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Abstract

本发明公开一种黄花蒿烟草醋液抑制根结线虫的应用。在其应用中，黄花蒿烟草醋液稀释25倍液至100倍液，根结线虫抑制率为90％～100％。

Figure 201710689170

The invention discloses the application of Artemisia annua tobacco vinegar for inhibiting root-knot nematodes. In its application, when the Artemisia annua tobacco vinegar solution is diluted from 25 times to 100 times, the inhibition rate of root knot nematodes is 90% to 100%.

Figure 201710689170

Description

Application of artemisia annua tobacco vinegar liquid in inhibition of root-knot nematodes
Technical Field
The invention belongs to the technical field of plant source material prevention and control of Chinese herbal medicine diseases, and particularly relates to application of artemisia annua tobacco vinegar liquid prepared by mixing artemisia annua straws and tobacco straws as a raw material to inhibition of root-knot nematodes.
Background
Artemisia annua Linn, also called as Artemisia annua L, is an annual herb of Artemisia of Compositae, widely distributed in various provinces of China, and is a traditional Chinese herbal medicine, the effective component artemisinin of the compound has different action mechanisms in the aspect of antimalarial than the traditional quinine antimalarial drugs, but although the artemisia annua plays an indispensable role in the medical health of human beings, because the growth and the propagation are extremely rapid, the habitat has strong adaptability, the east and the south provinces of China grow on the roadside, the wasteland, the hillside, the forest edge and the like, other provinces also grow on grassland, forest grassland, dry river valley, semi-desert, gravel slope land and the like, are also found on salinized soil, often germinate in large quantities in late spring and early summer, further develops into dominant weeds, and researches and developments on potential other utilization values of the weeds are also needed to prevent and control the damages of the weeds on farmlands, grasslands or forests.
After the tobacco leaves are harvested, a large amount of tobacco plant residues such as tobacco stems, tobacco roots and the like are remained, and if the tobacco plant residues are not removed in time, the environment of a tobacco planting area is easily polluted. The lignification degree of the tobacco (Nicotiana tabacum) straw is high, the tobacco is very difficult to decay, and the resource utilization of the tobacco (Nicotiana tabacum) straw is always a difficult problem. In recent years, the tobacco main production area has serious straw burning pollution. After tobacco is harvested, in order to treat the tobacco straws, the situation that the tobacco straws are burnt in partial areas to be dense and rolled is achieved, the treatment mode wastes precious natural resources, causes environmental pollution and multiple accidents, greatly threatens the traffic safety of highways and railways, the take-off and landing safety of civil aviation flights and the like, causes serious harm to human health and safety, and becomes a social problem. In order to solve the problem of relation among human resources, environment and development, the tobacco straws are efficiently and reasonably utilized, the potential utilization value of the tobacco straws is researched and developed, and the tobacco straws are also an important measure for reducing the negative influence of the tobacco straws.
The traditional Chinese medicine is a traditional Chinese medicinal material and is an important component of excellent Chinese culture. The Chinese herbal medicine belongs to the most important part of the Chinese herbal medicine, as the economic parts and the medicinal parts of most Chinese herbal medicines are roots of the Chinese herbal medicines, with the rapid increase of the planting area of medicinal plants and the domestication of wild resources of most varieties into the large-scale planting of cultivated varieties, the root knot nematode disease is one of the main diseases in the cultivation of crops, economic crops and Chinese herbal medicines, and occurs to different degrees every year. The overuse of chemical pesticides is increased for controlling diseases, so that the risk of qualified quality of Chinese herbal medicines and the friendliness to the environment are increased, which become main obstacles for the production of Chinese herbal medicines.
Through the literature search, the same public reports as the invention are not found.
Disclosure of Invention
The invention aims to overcome the defect that the artemisia annua tobacco vinegar liquid is not used for inhibiting root-knot nematodes in the prior art, so that the tobacco straw and the artemisia annua straw are utilized more.
In order to achieve the purpose, the technical scheme of the invention is as follows:
1. application of Artemisia annua tobacco vinegar liquid in inhibiting root-knot nematode is provided.
2. According to the application of the technical scheme 1, the artemisia annua tobacco vinegar liquid is prepared by mixing artemisia annua straws and tobacco straws and then preparing the vinegar liquid according to a conventional preparation method of plant vinegar liquid.
3. According to the application of the technical scheme 2, the artemisia annua straws and the tobacco straws are mixed into the artemisia annua straws: the tobacco straws are mixed according to the mass ratio of 1-2: 1-2.
4. According to the application of the technical scheme 3, the artemisia annua straws and the tobacco straws are mixed into the artemisia annua straws: the tobacco straws are mixed according to the mass ratio of 1: 1.
5. According to the application of the technical scheme 3, the artemisia annua straws and the tobacco straws are mixed into the artemisia annua straws: the tobacco straws are mixed according to the mass ratio of 2: 1.
6. According to the application of any one of the technical schemes 1 to 5, the artemisia annua tobacco vinegar liquid is 25-100 times liquid.
7. According to the application of the technical scheme 5, the artemisia annua tobacco vinegar liquid is 100 times liquid.
The invention has the beneficial effects that:
the application of the artemisia annua tobacco vinegar liquid in inhibiting the root-knot nematodes irrigates soil with perennial root-knot nematode diseases by using the artemisia annua tobacco vinegar liquid, and can effectively inhibit the root-knot nematodes. In the application, the artemisia annua tobacco vinegar liquid is diluted to 25-100 times, the inhibition rate of the root-knot nematodes is 90-100%, the artemisia annua straw single-dose vinegar liquid or the tobacco straw single-dose vinegar liquid is used for irrigating the soil with the root-knot nematode disease all the year round respectively, the vinegar liquid is diluted to 25-100 times, and the inhibition rates of the root-knot nematodes are 45-65% and 25-49% respectively.
Artemisia annua straw: the mass ratio of the tobacco straws is 2:1, the artemisia annua tobacco vinegar liquid has the best inhibition effect on the root-knot nematodes, the artemisia annua tobacco vinegar liquid is diluted by 25 times to 100 times, and the inhibition rate of the root-knot nematodes is 100%. When the artemisia annua vinegar liquid is diluted by 100 times, the inhibition rate of the root-knot nematode is 100%, and when the artemisia annua straw single-agent vinegar liquid is diluted by 100 times or the tobacco straw single-agent vinegar liquid is diluted by 100 times, the inhibition rate of the root-knot nematode is 45% and 25%, respectively.
Drawings
FIG. 1: schematic diagram of equipment for preparing artemisia annua tobacco vinegar.
The respective symbols in the drawings indicate in sequence: 1-a dry distillation kettle; 2-a heater; 3-an exhaust valve; 4-gas duct valve; 5, a condenser pipe air inlet guide pipe; 6-a condenser pipe; 7, a water outlet of the condensation pipe; 8-exhaust conduit of round bottom three-neck flask; 9-round bottom three-mouth flask; 10-round bottom flask; 11-water outlet of condensate tank; 12-a refrigerating water valve; 13-inlet pipe of condensation pipe; 14-condensate tank; 15-liquid inlet of round bottom three-mouth flask; 16-thermometer.
Detailed Description
The following examples are given without specific indication as conventional methods.
Example 1 preparation of Artemisia annua tobacco Vinegar
Firstly, preparing artemisia annua tobacco vinegar liquid by adopting conventional equipment for preparing vinegar liquid and a conventional preparation method of plant vinegar liquid, wherein artemisia annua straws: the artemisia annua tobacco vinegar liquid is prepared from the straws mixed according to the mass ratio of 1:1, 2:1 or 1:2 of the tobacco straws, and the method comprises the following steps:
a. cutting the artemisia annua straw and the tobacco straw into small sections (which can be cut into 5-8 cm small sections), and mixing the artemisia annua straw: mixing the short sections of the artemisia annua straws and the short sections of the tobacco straws in a mass ratio of 1:1 or 2:1 or 1:2, and then putting the mixture into a dry distillation kettle 1;
b. adding a sealing ring on the dry distillation kettle 1, installing a cover of the dry distillation kettle 1, and screwing a bolt of the dry distillation kettle;
c. opening an exhaust valve 3 on the dry distillation kettle 1;
d. turning on the heater 2 to heat the retort 1;
e. when the temperature of the thermometer 16 reaches 90-100 ℃, the exhaust valve 3 is closed;
f. opening a refrigerating water valve 12, and opening an air duct valve 4 on the dry distillation kettle 1;
g. controlling the heat of the heater 2, measuring the temperature of the dry distillation kettle 1 by using a thermometer 16 under normal pressure, and condensing and collecting all fractions at 90-300 ℃;
h. closing the gas-guide tube valve 4 on the dry distillation kettle 1, opening the exhaust valve 3 and closing the heater 2;
i. closing the refrigerating water valve 12, taking down the round-bottom three-neck flask 9, collecting the liquid (namely vinegar liquid) of the round-bottom three-neck flask 9, namely the artemisia annua tobacco vinegar liquid, and placing the artemisia annua tobacco vinegar liquid into a brown bottle for retention. Artemisia annua straw: mixing the tobacco straws in a mass ratio of 1:1 or 2:1 or 1:2 respectively to obtain three artemisia annua tobacco vinegar liquids: artemisia annua straw: the artemisia annua tobacco vinegar liquid with the tobacco straw mass ratio of 1:1 comprises the following components: the mass ratio of the tobacco straws is 2:1, artemisia annua tobacco vinegar liquid, artemisia annua straws: the mass ratio of the tobacco straws is 1:2, artemisia annua tobacco vinegar liquid.
Example 2 application experiment of Artemisia annua tobacco vinegar solution for inhibiting root-knot nematode
In the land parcel with root knot nematode disease and uniform disease all year round, the soil of the plant growth layer is taken, evenly mixed and equally divided into 6 parts, and the 6 parts of soil are respectively treated 1, 2 and 3, 4 and 5 and 6. Each treatment was repeated 3 times.
The treatment 1, the treatment 2 and the treatment 3 are experiments for inhibiting root-knot nematodes by using the artemisia annua tobacco vinegar liquid, and the artemisia annua tobacco vinegar liquids used in the treatment 1, the treatment 2 and the treatment 3 are different in that:
treatment 1: the artemisia annua tobacco vinegar liquid is prepared by mixing small artemisia annua straw segments and small tobacco straw segments according to the weight percentage of the artemisia annua straw: the tobacco straws are mixed according to the mass ratio of 1:1 and then are put into a dry distillation kettle 1, and then the artemisia annua tobacco vinegar is prepared according to the preparation method of the artemisia annua tobacco vinegar in the embodiment 1.
And (3) treatment 2: the used artemisia annua tobacco smoke is prepared by mixing small artemisia annua straw segments and small tobacco straw segments according to the following steps: the tobacco straws are mixed according to the mass ratio of 2:1 and then are put into a dry distillation kettle 1, and then the artemisia annua tobacco vinegar is prepared according to the preparation method of the artemisia annua tobacco vinegar in the embodiment 1.
And (3) treatment: the artemisia annua tobacco vinegar liquid is prepared by mixing small artemisia annua straw segments and small tobacco straw segments according to the weight percentage of the artemisia annua straw: mixing tobacco straws in a mass ratio of 1:2, putting the mixture into a dry distillation kettle 1, and preparing the artemisia annua tobacco vinegar liquid according to the preparation method of the artemisia annua tobacco vinegar liquid in the embodiment 1.
And (4) treatment: only single dose of artemisia annua straw vinegar is used for carrying out experiments.
And (4) treatment 5: only single dose of vinegar liquid of tobacco straw is used for carrying out the experiment.
The preparation method of the artemisia annua straw single-agent vinegar in the treatment 4 is the same as the preparation method of the artemisia annua tobacco vinegar in the example 1 except that the artemisia annua straw is only the artemisia annua straw in the dry distillation kettle 1 in the step a of the example 1.
The preparation method of the single-agent vinegar solution of the tobacco straws in the treatment 5 is the same as the preparation method of the artemisia annua tobacco vinegar solution in the example 1 except that the straws loaded into the retort 1 in the step a of the example 1 are only the tobacco straws.
Treatments 1, 2, 3, 4 and 5 the experimental operations were carried out as follows, except for the different vinegar solutions used as described above:
200ml of vinegar prepared by the preparation method is respectively prepared into 5000ml of solutions of 25 times, 50 times, 75 times, 100 times, 150 times and 200 times by using tap water according to the set 6 dilution gradients for later use. And (3) the prepared soil of the plot which has root knot nematode disease all year round and is uniformly developed is subpackaged in flowerpots, the israeled 208 tomatoes which are susceptible to the root knot nematode are planted, the prepared vinegar is used for irrigating (the amount of the vinegar for each irrigating treatment is equal), and the management is carried out according to a conventional planting method. After 8 weeks, the proportion of the root knots in the tomato root system in the whole root system, i.e. the percentage of the root knots, was investigated.
And (6) treatment: and (3) filling 1 part of equant soil into a flowerpot, planting the variety Israel 208 tomatoes susceptible to root-knot nematodes, and managing according to a conventional planting method. After 8 weeks, the proportion of the root knots in the tomato root system in the whole plant root system, namely the percentage of the root knots, is investigated.
And (3) test results: the vinegar solution with different dilution concentrations has an inhibition effect on the root-knot nematodes, but the inhibition effect is reduced along with the increase of the dilution times. Under the same dilution factor, the artemisia annua straws: the mass ratio of the tobacco straws is 2: the suppression effect was the best when 1 was used.
The inhibition rate of root-knot nematode is 1-root-knot percentage%.
The results are detailed in table 1.
TABLE 1 comparison of root knot nematode inhibition effect (percentage of root knot%) of Artemisia annua tobacco vinegar
Figure BDA0001376475420000051

Claims (6)

1.黄花蒿烟草醋液抑制根结线虫的应用，其特征在于：所述黄花蒿烟草醋液是通过将黄花蒿秸秆和烟草秸秆混合后按照植物醋液的常规制备方法制备得到的醋液；1. the application of Artemisia annua tobacco vinegar to inhibit root-knot nematode, it is characterized in that: described Artemisia annua tobacco vinegar is the vinegar prepared according to the conventional preparation method of plant vinegar after the Artemisia annua straw and tobacco straw are mixed; 所述黄花蒿烟草醋液的具体制备方法如下：The concrete preparation method of described Artemisia annua tobacco vinegar is as follows: a.将黄花蒿秸秆和烟草秸秆分别切成小段，按黄花蒿秸秆：将黄花蒿秸秆小段和烟草秸秆小段混合后装入干馏釜(1)中；A. Artemisia annua straw and tobacco straw are respectively cut into small sections, press Artemisia annua straw: the Artemisia annua straw section and tobacco straw section are mixed and loaded into the retort (1); b.加好干馏釜(1)上的密封圈，安装好干馏釜(1)的盖子，拧紧其螺栓；b. Add the sealing ring on the retort (1), install the cover of the retort (1), and tighten its bolts; c.打开干馏釜(1)上的排气阀门(3)；c. Open the exhaust valve (3) on the retort (1); d.打开加热器(2)，对干馏釜(1)进行加热；d. Turn on the heater (2) to heat the retort (1); e.在温度计(16)达到90℃-100℃时，关闭排气阀门(3)；e. When the thermometer (16) reaches 90℃-100℃, close the exhaust valve (3); f.打开制冷水阀门(12)，打开干馏釜(1)上的导气管阀门(4)；f. Open the refrigeration water valve (12), and open the air conduit valve (4) on the retort (1); g.控制加热器(2)的热度，常压下，利用温度计(16)测量干馏釜(1)的温度，冷凝收取90℃～300℃所有馏分；g. Control the heat of the heater (2), under normal pressure, use a thermometer (16) to measure the temperature of the retort (1), and condense and collect all fractions at 90°C to 300°C; h.关闭干馏釜(1)上的导气管阀门(4)，打开排气阀门(3)，关闭加热器(2)；h. Close the air conduit valve (4) on the retort (1), open the exhaust valve (3), and close the heater (2); i.关闭制冷水阀门(12)，取下圆底三口烧瓶(9)，收集圆底三口烧瓶(9)的醋液。i. Close the cooling water valve (12), remove the round-bottom three-neck flask (9), and collect the vinegar liquid in the round-bottom three-neck flask (9). 2.根据权利要求1所述的应用，其特征在于：所述将黄花蒿秸秆和烟草秸秆混合为按黄花蒿秸秆：烟草秸秆的质量比为1～2:1～2的比例混合。2 . The application according to claim 1 , wherein the mixing of Artemisia annua straw and tobacco straw is in a ratio of 1-2:1-2 according to the mass ratio of Artemisia annua straw:tobacco straw. 3 . 3.根据权利要求2所述的应用，其特征在于：所述将黄花蒿秸秆和烟草秸秆混合为按黄花蒿秸秆：烟草秸秆的质量比为1:1的比例混合。3 . The application according to claim 2 , wherein the mixing of Artemisia annua straw and tobacco straw is in a ratio of 1:1 according to the mass ratio of Artemisia annua straw:tobacco straw. 4 . 4.根据权利要求2所述的应用，其特征在于：所述将黄花蒿秸秆和烟草秸秆混合为按黄花蒿秸秆：烟草秸秆的质量比为2:1的比例混合。4 . The application according to claim 2 , wherein the mixing of Artemisia annua straw and tobacco straw is in a ratio of 2:1 according to the mass ratio of Artemisia annua straw:tobacco straw. 5 . 5.根据权利要求1至4中任一权利要求所述的应用，其特征在于：所述黄花蒿烟草醋液为25倍液至100倍液。5. according to the application described in any one of claim 1 to 4, it is characterized in that: described Artemisia annua tobacco vinegar liquid is 25 times of liquid to 100 times of liquid. 6.根据权利要求4所述的应用，其特征在于：所述黄花蒿烟草醋液为100倍液。6. application according to claim 4 is characterized in that: described Artemisia annua tobacco vinegar liquid is 100 times of liquids.
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CN102428975A (en) * 2011-12-16 2012-05-02 天长市中能国泰能源技术有限公司 Environment-friendly pesticide for rice
CN103724139A (en) * 2013-12-23 2014-04-16 青岛友铭辰生物技术有限公司 Biological pesticidal organic fertilizer and preparation method thereof
CN106135314A (en) * 2015-05-22 2016-11-23 龚玉 A kind of insecticidal formulation preventing and treating coccid and production method

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1387766A (en) * 2002-05-29 2003-01-01 吐尔逊古力·艾比布拉 Insecticide for preventing and controlling scale insects and its production method
CN101664046A (en) * 2009-09-23 2010-03-10 云南农业大学 Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof
CN102428975A (en) * 2011-12-16 2012-05-02 天长市中能国泰能源技术有限公司 Environment-friendly pesticide for rice
CN103724139A (en) * 2013-12-23 2014-04-16 青岛友铭辰生物技术有限公司 Biological pesticidal organic fertilizer and preparation method thereof
CN106135314A (en) * 2015-05-22 2016-11-23 龚玉 A kind of insecticidal formulation preventing and treating coccid and production method

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