CN107372634A - Artemisia annua tobacco vinegar liquid - Google Patents

Abstract

The invention discloses an Artemisia annua tobacco vinegar liquid. With 25-fold to 100-fold liquid of the Artemisia annua tobacco vinegar liquid, the inhibition rate against Fusarium oxysporum reaches 79.17%-100%. The root-knot nematode inhibition rate reaches 90% to 100% by watering the soil where root-knot nematode occurs all year round.

Description

Artemisia annua Tobacco Vinegar

technical field

The invention belongs to the technical field of preventing and controlling root-knot nematode diseases and root rot diseases of crops and Chinese herbal medicines by plant source materials. The invention relates to a vinegar solution prepared by mixing Artemisia annua stalks and tobacco stalks, and the vinegar solution can inhibit root-knot nematodes and root rot pathogens of Chinese herbal medicines.

Background technique

Artemisia annua Linn., also known as Artemisia annua, is an annual herb of the genus Artemisia in the Asteraceae family. It is widely distributed in various provinces in China. It is a traditional Chinese herbal medicine. Antimalarial drugs have different mechanisms of action. However, although Artemisia annua has made an indispensable contribution to human medical health, due to its rapid growth and reproduction and strong habitat adaptability, it grows in roadsides, wastelands, hillsides, forest edges, etc. in eastern and southern provinces of my country. Provinces also grow in grasslands, forest grasslands, dry river valleys, semi-deserts, and gravel slopes, etc., and are also found on saline soils. They often germinate in large numbers in late spring and early summer, and then develop into dominant weeds. In order to prevent and control other weeds As the harm of weeds to farmland, grassland or forest, it is also necessary to research and develop its other potential use values.

After the tobacco leaves are harvested, a large amount of tobacco plant residues such as tobacco stalks and tobacco roots remain. If they are not removed in time, it is easy to pollute the environment of tobacco growing areas. Tobacco (Nicotiana tabacum) straw has a high degree of lignification and is very difficult to decompose. Its resource utilization has always been a difficult problem. In recent years, there has been serious pollution from straw burning in major tobacco producing areas. Every time after the tobacco is harvested, in order to deal with the tobacco stalks, some areas have reached the situation of billowing smoke. This treatment method not only wastes precious natural resources, causes environmental pollution, but also causes frequent accidents. The security and take-off and landing safety of civil aviation flights pose a great threat, and cause serious harm to human health and safety, which has become a major social problem. In order to solve the relationship between human resources, environment and development, efficient and rational use of tobacco stalks, research and development of its potential use value are also important measures to reduce its negative impact.

Traditional Chinese medicine is a traditional Chinese medicinal material and an important part of Chinese excellent culture. Chinese herbal medicine is the most important part of Chinese medicinal materials. Since the economic and medicinal parts of most Chinese herbal medicines are the roots of Chinese herbal medicines, with the rapid increase in the planting area of medicinal plants and the domestication of most varieties of wild resources into large-scale planting of cultivated varieties, Root rot has evolved into the most serious disease that directly affects the yield, quality and commodity value of medicinal materials, and greatly affects the value of Chinese herbal medicines. Furthermore, the problem of Chinese herbal medicine diseases is becoming more and more prominent. Because the microbial composition in the soil is very complex, there are many types of pathogens involved. Fungi, bacteria, and nematodes can cause the roots of Chinese herbal medicines to rot. Among them, fungi are the most serious and can cause root rots of various Chinese herbal medicines, such as Fusarium oxysporum (Fusarium oxysporum) can cause root rot of Chinese herbal medicines such as Lycium barbarum, Panax ginseng, Polygonatum odoratum, Salvia miltiorrhiza, Panax notoginseng, and is one of the most harmful pathogens. The annual incidence rate of root rot of Panax notoginseng is 5% to 20%, and in severe cases it reaches 70%. The incidence rate of root rot of Angelica dahurica is about 30%, and sometimes it reaches 80%. In order to control its diseases, the excessive use of chemical pesticides has been increased, which in turn aggravates the risk of qualified Chinese herbal medicines and the friendliness to the environment, which has become a major obstacle to the production of Chinese herbal medicines.

Root-knot nematode (Meloidogyne spp.) is a highly specialized omnivorous phytopathogenic nematode that occurs in the underground parts of plants. Root-knot nematode is one of the main diseases in the cultivation of crops, economic crops and Chinese herbal medicine, and it occurs to varying degrees every year. Since the economic and medicinal parts of most Chinese herbal medicines are the roots of Chinese herbal medicines, with the rapid increase in the planting area of medicinal plants and the domestication of wild resources of most varieties into large-scale planting of cultivated varieties, the root-knot nematode disease of medicinal plants Occurrence is increasing. In order to control its diseases, the excessive use of chemical pesticides has been increased, which has aggravated the risk of qualified Chinese herbal medicines and the unfriendly nature of the environment, which has become one of the main obstacles to the production of Chinese herbal medicines.

Through document search, do not see the public report identical with the present invention.

Contents of the invention

The object of the present invention is to provide an Artemisia annua tobacco vinegar product capable of effectively inhibiting root-knot nematodes and root rot pathogens of Chinese herbal medicines, so that tobacco stalks and Artemisia annua stalks can be more utilized.

To achieve the above object, the technical scheme of the present invention is as follows:

1. Artemisia annua Tobacco Vinegar.

2. The Artemisia annua tobacco vinegar liquid according to technical scheme 1, said Artemisia annua tobacco vinegar liquid is prepared according to the conventional preparation method of plant vinegar liquid after mixing Artemisia annua straw and tobacco straw.

3. According to the Artemisia annua tobacco vinegar liquid described in technical solution 2, the Artemisia annua straw and the tobacco straw are mixed in a mass ratio of 1-2:1-2.

4. According to the Artemisia annua tobacco vinegar liquid described in technical solution 3, the Artemisia annua straw and tobacco straw are mixed in a ratio of 1:1 by mass ratio of Artemisia annua straw:tobacco straw.

5. According to the Artemisia annua tobacco vinegar liquid described in technical solution 3, the Artemisia annua straw and tobacco straw are mixed in a ratio of 2:1 by mass ratio of Artemisia annua straw:tobacco straw.

The present invention utilizes Artemisia annua tobacco vinegar liquid for the first time to suppress root rot pathogenic fungus Fusarium oxysporum (Fusarium oxysporum) and root-knot nematode of Panax notoginseng.

Using the Artemisia annua tobacco vinegar liquid of the present invention to irrigate the soil where root-knot nematode occurs all year round can effectively inhibit root-knot nematodes, and the Artemisia annua tobacco vinegar liquid is diluted 25 times to 100 times, and the root-knot nematode inhibition rate is 90 % to 100%, and the single-dose vinegar solution of Artemisia annua straw or tobacco straw single-dose vinegar solution is used to irrigate the soil where root-knot nematode disease occurs all the year round, and the vinegar solution is diluted 25 times to 100 times. The inhibition rate is 45%～65%, 25%～49% respectively, and the inhibitory effect of the Artemisia annua tobacco vinegar liquid of the present invention on root-knot nematodes is respectively increased by 53.85% compared with the single-dose vinegar liquid of Artemisia annua straw or the single-dose vinegar liquid of tobacco straw. %~100%, 104.08%~260%.

The Artemisia annua straw: tobacco straw mass ratio of the present invention Artemisia annua tobacco vinegar liquid with a mass ratio of 2:1 has the best inhibitory effect on root-knot nematodes, and the inhibition rate of root-knot nematodes is 100% when diluted 25 times to 100 times. However, when the single-dose vinegar solution of Artemisia annua stalks is diluted 100 times or the single-dose vinegar solution of tobacco stalks is diluted 100 times, the inhibition rate of root-knot nematodes is 45% and 25% respectively. Compared with the 100-fold solution of single-dose vinegar solution of Artemisia annua straw or the 100-fold solution of single-dose vinegar solution of tobacco straw, the inhibitory effect was increased by 122% and 300%, respectively.

When the Artemisia annua tobacco vinegar liquid of the present invention is diluted 25 times to 100 times, the inhibition rate of the pathogenic fungus Fusarium oxysporum of Radix Notoginseng root rot reaches 79.17% to 100%. The 100-fold liquid has an increased inhibition rate of 78.15% to 111.77% against Fusarium oxysporum. The inhibitory rate of Fusarium oxysporum increased by 111.12% to 139.98% when diluted 25-fold to 100-fold compared with a single-dose vinegar solution of tobacco stalks.

The Artemisia annua straw: tobacco stalk mass ratio of the present invention is 2:1 Artemisia annua tobacco vinegar to inhibit the pathogenic fungus of Radix Notoginseng root rot, and when diluted 100 times, the inhibition rate of inhibiting the pathogenic fungus of Radix Notoginseng root rot Fusarium oxysporum is 98.61%, while the inhibitory rates of using Artemisia annua stalk single-dose vinegar and tobacco stalk single-dose vinegar respectively to inhibit the pathogenic fungus Fusarium oxysporum of Radix Notoginseng root rot were 44.44%, 37.5%, Artemisia annua straw of the present invention: tobacco stalk The 100-fold solution of Artemisia annua tobacco vinegar with a mass ratio of 2:1 has a better inhibitory effect on the pathogenic fungus Fusarium oxysporum of Panax notoginseng root rot than single-dose vinegar solution 100-fold solution of Artemisia annua straw or single-dose vinegar solution 100 times solution of tobacco straw They have increased by 121.89% and 162.96% respectively.

Compared with the single-dose vinegar solution of Artemisia annua straw or the single-dose vinegar solution of tobacco stalks, the present invention's Artemisia annua tobacco vinegar solution has produced unexpected technical effects on inhibition of root-knot nematodes and pathogenic fungi of Chinese herbal medicine root rot.

Description of drawings

Figure 1: Schematic diagram of the equipment for preparing Artemisia annua tobacco vinegar.

The marks in the figure indicate in turn: 1—retort; 2—heater; 3—exhaust valve; 4—air pipe valve; 5—intake pipe of condensation pipe; 6—condensation pipe; 7—water outlet of condensation pipe; 8 —Exhaust conduit of the three-neck round-bottom flask; 9—Three-neck round-bottom flask; 10—Round-bottom flask; 11-Condensation tank outlet; 12-Cooling water valve; 13-Condenser inlet pipe; Liquid inlet of round bottom three-neck flask; 16—thermometer.

detailed description

The following examples are conventional methods without special instructions.

The preparation of embodiment 1 Artemisia annua tobacco vinegar liquid

1. The Artemisia annua Tobacco Vinegar is prepared by using the conventional equipment for preparing vinegar and the conventional preparation method of plant vinegar, and the mass ratio of Artemisia annua straw:tobacco straw is mixed in the ratio of 1:1 or 2:1 or 1:2 respectively Straw preparation Artemisia annua tobacco vinegar liquid, all carry out according to the following steps:

a. Cut Artemisia annua stalks and tobacco stalks into small sections respectively (can be cut into small sections of 5cm-8cm), according to the mass ratio of Artemisia annua stalks:tobacco stalks in the ratio of 1:1 or 2:1 or 1:2, the The small pieces of Artemisia annua stalks and the small pieces of tobacco stalks are mixed and loaded into the retort 1;

b. Add the sealing ring on the retort 1, install the cover of the retort 1, and tighten the bolts;

c. Open the exhaust valve 3 on the retort 1;

d. Turn on the heater 2 to heat the retort 1;

e. When the thermometer 16 reaches 90°C-100°C, close the exhaust valve 3;

f. Open the cooling water valve 12, and open the gas guide valve 4 on the retort 1;

g. Control the heat of the heater 2, measure the temperature of the retort 1 with a thermometer 16 under normal pressure, and condense and collect all fractions at 90°C to 300°C;

h. Close the gas guide valve 4 on the retort 1, open the exhaust valve 3, and close the heater 2;

i. Close the cooling water valve 12, take off the three-necked round-bottomed flask 9, and the liquid (ie vinegar liquid) collected by the three-necked round-bottomed flask 9 is the Artemisia annua tobacco vinegar liquid of the present invention, which is placed in a brown bottle and reserved. The mass ratio of Artemisia annua straw:tobacco straw is 1:1 or 2:1 or 1:2 respectively to obtain three kinds of Artemisia annua tobacco vinegar: Artemisia annua straw:Tobacco straw mass ratio is 1:1 Tobacco vinegar liquid, Artemisia annua straw: tobacco straw mass ratio of 2:1 Artemisia annua tobacco vinegar liquid, Artemisia annua straw: tobacco straw mass ratio of 1:2 Artemisia annua tobacco vinegar liquid.

Example 2 Application Experiment of Artemisia annua Tobacco Vinegar Liquid in Inhibiting Root-knot Nematodes

In plots where root-knot nematode disease occurs all year round and the incidence is uniform, the soil in the plant growth layer is taken, mixed evenly and divided into 6 parts, and the 6 parts of soil are respectively treatment 1, treatment 2, treatment 3, treatment 4, treatment 5, treatment 6. Each treatment was repeated 3 times.

Treatment 1, treatment 2 and treatment 3 are all the experiments of suppressing root-knot nematodes with the Artemisia annua tobacco vinegar liquid of the present invention, and the differences in the used Artemisia annua tobacco vinegar liquid for treatment 1, treatment 2 and treatment 3 are:

Treatment 1: the used Artemisia annua tobacco vinegar liquid is mixed with Artemisia annua stalks and tobacco stalks at a ratio of 1:1 mass ratio of Artemisia annua stalks:tobacco stalks, then put into retort 1, and then according to Example 1 The preparation method of Artemisia annua tobacco vinegar liquid is prepared to obtain Artemisia annua tobacco vinegar liquid.

Treatment 2: the used Artemisia annua tobacco smoke is mixed with Artemisia annua stalks and tobacco stalks at a ratio of 2:1 mass ratio of Artemisia annua stalks:tobacco stalks, then put into retort 1, and then as described in Example 1 The preparation method of Artemisia annua tobacco vinegar liquid is prepared to obtain Artemisia annua tobacco vinegar liquid.

Treatment 3: the used Artemisia annua tobacco vinegar liquid is mixed with Artemisia annua stalks and tobacco stalks at a ratio of 1:2 by mass of Artemisia annua stalks:tobacco stalks, then put into retort 1, and then according to Example 1 The preparation method of Artemisia annua tobacco vinegar liquid is prepared to obtain Artemisia annua tobacco vinegar liquid.

Treatment 4: The experiment was carried out only with single-dose vinegar solution of Artemisia annua straw.

Treatment 5: the experiment was carried out with only tobacco stalk single-dose vinegar solution.

The preparation method of Artemisia annua straw single-dose vinegar solution in treatment 4 is except that in step a of Example 1, the straw loaded into retort 1 is only Artemisia annua straw, the rest of the operations are the same as the preparation of Artemisia annua tobacco vinegar solution described in Example 1 The method is the same.

The preparation method of tobacco stalk single-dose vinegar solution in treatment 5 is the same as the preparation method of the Artemisia annua tobacco vinegar solution described in Example 1, except that the stalks loaded into retort 1 in step a in Example 1 are only tobacco stalks .

Treatment 1, treatment 2, treatment 3, treatment 4 and treatment 5 except the vinegar solution used as mentioned above are different, all carry out experimental operation according to the following method:

Utilize the above-mentioned preparation method to prepare 200ml of vinegar liquid, according to the 6 dilution gradients set up, use tap water to prepare 5000ml of 25 times liquid, 50 times liquid, 75 times liquid, 100 times liquid, 150 times liquid and 200 times liquid respectively ,spare. The prepared root-knot nematode disease occurs all year round and the soil of the evenly morbid plot is subpacked in flower pots, and the susceptible root-knot nematode variety Israel 208 tomato is planted, watered with the prepared vinegar solution (each handles watering The amount of vinegar solution is equal), and managed according to the conventional planting method. After 8 weeks, investigate the ratio of the root knots in the tomato root system to the entire root system, that is, the root knot percentage.

Treatment 6: Put an aliquot of the soil in a flower pot, plant the root-knot nematode-susceptible variety Israel 208 tomato, and manage it according to the conventional planting method. After 8 weeks, investigate the ratio of the root knots in the tomato root system to the root system of the whole plant, that is, the root knot percentage.

Test results: Vinegar solutions with different dilution concentrations have inhibitory effects on root-knot nematodes, but the inhibitory effects decrease with the increase of dilution times. Under the same dilution factor, the inhibitory effect was the best when the mass ratio of Artemisia annua straw:tobacco straw was 2:1.

Root-knot nematode inhibition rate%=1-root-knot percentage%.

The experimental results of Example 2 are shown in Table 1.

Table 1 Comparison of the effects of Artemisia annua Tobacco Vinegar Liquid on inhibiting root-knot nematodes (root-knot percentage %)

Example 3 Application Experiment of Artemisia annua Tobacco Vinegar Liquid Inhibiting Radix Notoginseng Root Rot Pathogenic Fungus

The notoginseng root rot pathogenic fungus used in embodiment 3 is Fusarium oxysporum (Fusarium oxysporum), and its isolation and identification culture method refers to [Fang Zhongda editor-in-chief. Plant disease research methods (third edition). 2004, Beijing: China Agriculture Press; CBooth.The genus Fusarium.Commonwealth Agricultura,1971; Wei Jingchao. Handbook of Fungal Identification. Shanghai: Shanghai Science and Technology Press, 1979].

Treatment 1, treatment 2 and treatment 3 are all experimented with the Artemisia annua tobacco vinegar liquid, and the difference between treatment 1, treatment 2 and treatment 3 is:

Treatment 1: the used Artemisia annua tobacco vinegar liquid is mixed with Artemisia annua stalks and tobacco stalks at a ratio of 1:1 mass ratio of Artemisia annua stalks:tobacco stalks, then put into retort 1, and then according to Example 1 Artemisia annua tobacco vinegar liquid prepared by the preparation method of Artemisia annua tobacco vinegar liquid.

Treatment 2: the used Artemisia annua tobacco smoke is mixed with Artemisia annua stalks and tobacco stalks at a ratio of 2:1 mass ratio of Artemisia annua stalks:tobacco stalks, then put into retort 1, and then as described in Example 1 The Artemisia annua tobacco vinegar liquid prepared by the preparation method of the Artemisia annua tobacco vinegar liquid.

Treatment 3: the used Artemisia annua tobacco vinegar liquid is mixed with Artemisia annua stalks and tobacco stalks at a ratio of 1:2 by mass of Artemisia annua stalks:tobacco stalks, then put into retort 1, and then according to Example 1 Artemisia annua tobacco vinegar liquid prepared by the preparation method of Artemisia annua tobacco vinegar liquid.

Treatment 4: The experiment was carried out only with single-dose vinegar solution of Artemisia annua straw.

Treatment 5: the experiment was carried out with only tobacco stalk single-dose vinegar solution.

The preparation method of Artemisia annua straw single-dose vinegar solution in treatment 4 is except that in step a of Example 1, the straw loaded into retort 1 is only Artemisia annua straw, the rest of the operations are the same as the preparation of Artemisia annua tobacco vinegar solution described in Example 1 The method is the same.

The preparation method of tobacco stalk single-dose vinegar solution in treatment 5 is the same as the preparation method of the Artemisia annua tobacco vinegar solution described in Example 1, except that the stalks loaded into retort 1 in step a in Example 1 are only tobacco stalks .

Treatment 1, treatment 2, treatment 3, treatment 4 and treatment 5 except the vinegar solution used as mentioned above are different, all carry out experimental operation according to the following method:

Utilize the vinegar solution prepared by the above method, according to the mass ratio of vinegar solution and PDA medium as follows, prepare the dilution ratio of vinegar solution as 25 times, 50 times, 75 times, 100 times, 150 times and 200 times respectively The PDA medium containing vinegar solution, i.e. the mass ratio of vinegar solution:PDA medium=1:24, the mass ratio of vinegar solution:PDA medium=1:49, the mass ratio of vinegar solution:PDA medium=1 :74, the mass ratio of vinegar solution:PDA medium=1:99, the mass ratio of vinegar solution:PDA medium=1:149, the mass ratio of vinegar solution:PDA medium=1:199. Each kind of vinegar liquid prepares the above-mentioned 25 times of liquid, 50 times of liquid, 75 times of liquid, 100 times of liquid, 150 times of liquid and 200 times of liquid, the PDA culture medium containing vinegar liquid of 6 different dilution multiples of vinegar liquid, respectively packs Put into a Petri dish, and each Petri dish is filled with 10 ml of PDA medium containing vinegar solution.

Under sterile conditions, use the same type of hole puncher (0.5cm, sterilized) to make holes on the edge of the colonies of Fusarium oxysporum identified by culture and purification, and use a scalpel to pick out the colonies punched with the hole puncher The Fusarium oxysporum colonies of the same size and activity were transferred to the above-mentioned petri dishes containing vinegar-containing PDA medium, and one Fusarium oxysporum colony was transferred to each petri dish, and the petri dish was covered. Put it in an incubator for 5 days, measure and record the growth diameter of the colony, and calculate the inhibitory effect.

Treatment 6 is a blank control experiment. Treatment 6: Under aseptic conditions, transfer 1 Fusarium oxysporum colony into a petri dish containing only 10 ml of PDA medium according to the above method, cover the petri dish, and directly place it in the incubator for 5 days. Measure and record the colony growth diameter.

Each treatment was repeated 3 times.

Test results: Vinegar solutions with different dilution concentrations had inhibitory effects on Fusarium oxysporum, but the inhibitory effects decreased with the increase of dilution times. Under the same dilution factor, the inhibition effect of Artemisia annua tobacco vinegar liquid was the best when the mass ratio of Artemisia annua straw:tobacco straw was 2:1.

The experimental results of Example 3 are shown in Table 2.

Table 2 Comparison of the effects of Artemisia annua Tobacco Vinegar on inhibiting pathogenic fungi of Radix Notoginseng root rot (colony diameter cm)

Fusarium oxysporum inhibition rate calculation formula:

Inhibition rate of Fusarium oxysporum=[︱diameter of treated colony-diameter of untreated colony︱/diameter of untreated colony]X100%.

Table 2 shows that:

Treatment 1: Artemisia annua straw: Tobacco stalk mass ratio of 1:1 with Artemisia annua tobacco vinegar diluted 25 times, 50 times, 75 times, 100 times, 150 times and 200 times against Fusarium oxysporum The inhibition rates were 88.89%, 88.89%, 87.5%, 83.33%, 83.33%, and 81.94%, respectively.

Treatment 2: Artemisia annua straw: Tobacco stalk mass ratio of 2:1 Artemisia annua tobacco vinegar diluted 25 times, 50 times, 75 times, 100 times, 150 times and 200 times against Fusarium oxysporum The inhibition rates were 100%, 100%, 98.61%, 98.61%, 97.22%, and 97.22%, respectively.

Treatment 3: Artemisia annua straw: Tobacco stalk mass ratio of 1:2 with Artemisia annua tobacco vinegar diluted 25 times, 50 times, 75 times, 100 times, 150 times and 200 times against Fusarium oxysporum The inhibition rates were 86.11%, 86.11%, 86.11%, 79.17%, 75.00%, and 72.22%, respectively.

Treatment 4: The inhibition rates of Artemisia annua straw single-dose vinegar solution diluted 25 times, 50 times, 75 times, 100 times, 150 times and 200 times against Fusarium oxysporum were 47.22%, 47.22%, 47.22% respectively %, 44.44%, 44.44%, 37.50%.

Treatment 5: Tobacco stalk single-dose vinegar dilution 25 times, 50 times, 75 times, 100 times, 150 times and 200 times the inhibition rate of Fusarium oxysporum were 41.67%, 41.67%, 41.67% , 37.50%, 26.39%, 23.61%.

When the Artemisia annua tobacco vinegar was diluted 25 times to 100 times, the inhibition rate of Fusarium oxysporum, the pathogenic fungus of Panax notoginseng root rot, reached 79.17% to 100%, while the single dose vinegar solution of Artemisia annua straw was diluted 25 times to 100 times The inhibitory rate of liquid to Fusarium oxysporum is 44.44%～47.22%. When Artemisia annua tobacco vinegar is diluted 25 times to 100 times, it is more effective than Artemisia annua straw single agent vinegar diluted 25 times to 100 times against Fusarium oxysporum. The bacterial inhibition rate increased by 78.15% to 111.77%. However, the inhibition rate of tobacco stalk single-dose vinegar diluted from 25 times to 100 times against Fusarium oxysporum was 37.50% to 41.67%. The inhibition rate of Fusarium oxysporum was increased by 111.12% to 139.98% when the solution was diluted from 25 times to 100 times.

Artemisia annua straw: Tobacco straw mass ratio of 2:1 Artemisia annua tobacco vinegar has the best inhibitory effect on the root rot pathogenic fungus Fusarium oxysporum of Panax notoginseng, and the inhibitory effect on the root rot fungus Fusarium oxysporum of Panax notoginseng reaches 98.61% to 100%, when it is diluted 100 times, the inhibitory effect on the pathogenic fungus Fusarium oxysporum of Panax notoginseng root rot reaches 98.61%. The 100-fold liquid had an inhibitory rate of 44.44% and 37.5% respectively against Fusarium oxysporum, the pathogenic fungus of Panax notoginseng root rot, which was 121.89% higher than the 100-fold liquid vinegar liquid of Artemisia annua straw alone; The 100-fold liquid vinegar solution has an increase of 162.96% in the inhibition rate of Fusarium oxysporum.

Claims (5)

1. Artemisia annua Tobacco Vinegar. 2. The Artemisia annua tobacco vinegar liquid according to claim 1, characterized in that: said Artemisia annua tobacco vinegar liquid is prepared according to the conventional preparation method of vegetable vinegar after mixing Artemisia annua stalks and tobacco stalks . 3. The Artemisia annua tobacco vinegar liquid according to claim 2, characterized in that: said Artemisia annua stalks and tobacco stalks are mixed so that the mass ratio of Artemisia annua stalks:tobacco stalks is 1-2:1-2 mix. 4. The Artemisia annua tobacco vinegar liquid according to claim 3, characterized in that: said Artemisia annua straw and tobacco straw are mixed in a mass ratio of Artemisia annua straw:tobacco straw at a ratio of 1:1. 5 . The Artemisia annua tobacco vinegar liquid according to claim 3 , characterized in that: said Artemisia annua straw and tobacco straw are mixed in a mass ratio of Artemisia annua straw:tobacco straw at a ratio of 2:1.