CN113973851A - Chinese herbal medicine ointment for preventing and treating fruit tree rot disease and preparation method and application thereof - Google Patents
Chinese herbal medicine ointment for preventing and treating fruit tree rot disease and preparation method and application thereof Download PDFInfo
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protection of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/14—Celastraceae [Staff-tree or Bittersweet family], e.g. spindle tree, bittersweet or thunder god vine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
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Abstract
The invention relates to the technical field of agricultural disease control, in particular to a Chinese herbal ointment for controlling fruit tree canker, a preparation method and application thereof, wherein the Chinese herbal ointment is prepared from 0.8g/mL perilla leaf ethanol extract, 95% corydalis bungeana ethanol extract, 95% cassia twig ethanol extract, 95% sophora flavescens ethanol extract, 95% tripterygium wilfordii ethanol extract, surfactant sodium dodecyl sulfate, indoleacetic acid hot melt adhesive matrix and borneol. The invention can obviously reduce the disease index and incidence of apple tree canker, obviously improve the prevention effect, obviously improve the apple tree defensive enzyme activity, obviously increase the expression level of the course-related protein genes PR5 and PR8, obviously improve the content of free proline, obviously reduce the membrane peroxidation degree, and obviously enhance the resistance of the apple tree to the canker.
Description
Technical Field
The invention relates to the technical field of agricultural disease control, in particular to a Chinese herbal medicine ointment for controlling fruit tree rot and a preparation method and application thereof.
Background
Apple tree canker, commonly known as rotten skin disease and stinking skin disease, is an important branch disease of apple trees. The disease mainly damages fruiter trees, and in recent years, the disease also damages saplings and seedlings, thereby bringing serious threat to the production of fruit trees.
At present, the common medicaments for apple tree canker mainly comprise carbendazim medicament mud, tebuconazole, flusilazole, methionyl acetate and the like, and good control effect is achieved. However, the large amount and long-term use of chemical agents not only poses serious threat to human health, but also often causes environmental pollution and drug resistance of pathogenic bacteria.
Disclosure of Invention
In order to solve the problems, the invention provides a Chinese herbal medicine ointment for preventing and treating fruit tree rot, a preparation method and application thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
a Chinese herbal ointment for preventing and treating fruit tree canker is prepared from folium Perillae ethanol extract 0.8g/mL, herba Violae 95% ethanol extract, ramulus Cinnamomi 95% ethanol extract, radix Sophorae Flavescentis 95% ethanol extract, radix Tripterygii Wilfordii 95% ethanol extract, surfactant sodium dodecyl sulfate, indoleacetic acid hot melt adhesive matrix and Borneolum Syntheticum.
Preferably, the dosage of each raw material is as follows according to the mass ratio:
0.8g/mL of perilla leaf ethanol extract 1-9, 1-2.5 of corydalis 95% ethanol extract, 1-3 of cassia twig 95% ethanol extract, 1-3 of radix sophorae flavescentis 95% ethanol extract, 1-2.5 of tripterygium wilfordii 95% ethanol extract, 2-3 of surfactant lauryl sodium sulfate, 0.5-1 of borneol, 4-10 ppm of indoleacetic acid in total mass, and 20-7 of proportion of hot melt adhesive matrix to medicament.
Further, the corydalis bungeana ethanol extract with the concentration of 95%, the cassia twig ethanol extract with the concentration of 95%, the kuh-seng ethanol extract with the concentration of 95% and the tripterygium wilfordii ethanol extract with the concentration of 95% are respectively 100g, 1000ml of ethanol solution with the concentration of 95% is respectively added, the extraction is carried out for 2h at the temperature of 40 ℃, the extraction is carried out for 3 times, and the extracting solution is obtained after mixing.
The invention also discloses a preparation method of the Chinese herbal medicine ointment for preventing and treating the rot disease of the fruit trees, which comprises the following steps:
taking 1-9 parts of 0.8g/mL perilla leaf ethanol extract, 1-2.5 parts of corydalis bungeana 95% ethanol extract, 1-3 parts of cassia twig 95% ethanol extract, 1-3 parts of radix sophorae flavescentis 95% ethanol extract and 1-2.5 parts of tripterygium wilfordii 95% ethanol extract according to the mass ratio, sieving with an 80-mesh sieve, mixing, adding 2-3 parts of surfactant, stirring uniformly, adding 4-10 parts per million of indoleacetic acid, and stirring uniformly to obtain an agent A for later use;
and (3) putting the hot melt adhesive into a melter to be melted or heating the hot melt adhesive to 130 ℃ by using an iron pan to be completely melted, turning to slow fire, pouring the agent A, uniformly stirring, adding 0.5-1 part of borneol, spreading the ointment immediately after stirring, putting the plaster on kraft paper, and putting the plaster on the surface of the plaster with oil covered by silicone oil paper.
The Chinese herbal medicine paste for preventing and treating the fruit tree rot can be used for preventing and treating the fruit tree rot, and comprises the following steps:
treating the lesion spots: and (3) cutting the disease spots into a cutting path of 0.8-1 cm by a knife, wherein the cutting path is along the growth direction of the trunk or the branches, the depth of the cutting groove reaches xylem, the range of the nick exceeds the range of the disease spots by 1-2 cm, the plaster is stuck to the treated disease spots and fixed, and the plaster is replaced once every 5-7 days, so that a good prevention and treatment effect can be achieved for three times.
The invention has the strongest bacteriostasis to the fungus of the Humicola melanocortin, can obviously reduce the disease index and incidence of the apple tree canker, obviously improves the prevention and treatment effect, obviously improves the defensive enzyme activity of the apple tree, obviously increases the expression level of the course-related protein genes PR5 and PR8, obviously improves the content of free proline, obviously reduces the peroxidation degree of membranous substances, and can obviously enhance the resistance of the apple tree to the canker.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described in detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The invention provides a Chinese herbal medicine ointment for preventing and treating fruit tree rot, which selects 95% ethanol extract of perilla leaf part of 0.8g/mL as a main effective component, simultaneously selects ethanol extracts of corydalis bungeana, cassia twig, radix sophorae flavescentis and tripterygium wilfordii, surfactant sodium dodecyl sulfate and indoleacetic acid, and auxiliary materials are hot melt adhesive matrix and borneol, wherein the dosage of each raw material is as follows by mass ratio:
0.8g/mL of perilla leaf ethanol extract 1-9, 1-2.5 of viola 95% ethanol extract, 1-3 of cassia twig 95% ethanol extract, 1-3 of radix sophorae flavescentis 95% ethanol extract, 1-2.5 of tripterygium wilfordii 95% ethanol extract, 2-3 of surfactant sodium dodecyl sulfate, 0.5-1 of borneol, 4-10 ppm of indoleacetic acid in total mass and 20-7 of ratio of hot melt adhesive matrix to medicament, wherein 0.8g/mL of perilla leaf ethanol extract, bunge corydalis herb, cassia twig, radix sophorae flavescentis and tripterygium wilfordii ethanol extract has strong inhibitory effect on fruit tree rot, indoleacetic acid can promote cell division and callus formation, and surfactant sodium dodecyl sulfate can enhance Chinese herbal medicine active substance permeation.
The preparation method comprises the following steps:
taking 1-9 parts of 0.8g/mL perilla leaf ethanol extract, 1-2.5 parts of corydalis bungeana 95% ethanol extract, 1-3 parts of cassia twig 95% ethanol extract, 1-3 parts of radix sophorae flavescentis 95% ethanol extract and 1-2.5 parts of tripterygium wilfordii 95% ethanol extract according to the mass ratio, sieving with an 80-mesh sieve, mixing, adding 2-3 parts of surfactant, stirring uniformly, adding 4-10 parts per million of indoleacetic acid, and stirring uniformly to obtain an agent A for later use;
and (3) putting the hot melt adhesive into a melter to be melted or heating the hot melt adhesive to 130 ℃ by using an iron pan to be completely melted, turning to slow fire, pouring the agent A, uniformly stirring, adding 0.5-1 part of borneol, spreading the ointment immediately after stirring, putting the plaster on kraft paper, and putting the plaster on the surface of the plaster with oil covered by silicone oil paper.
When the Chinese herbal medicine paste for preventing and treating the rot disease of the fruit trees is used, the method comprises the following steps:
s1, brushing or spraying a trunk with 2.5g of oligomale-rot enzyme and 5KG of water in 3-4 months for prevention;
s2, treatment of lesion spots: and (3) cutting the disease spots into a cutting path of 0.8-1 cm by a knife, wherein the cutting path is along the growth direction of the trunk or the branches, the depth of the cutting groove reaches xylem, the range of the nick exceeds the range of the disease spots by 1-2 cm, the plaster is stuck to the treated disease spots and fixed, and the plaster is replaced once every 5-7 days, so that a good prevention and treatment effect can be achieved for three times.
The field application detection finds that: the Chinese herbal medicine ointment can obviously reduce the disease index and incidence of apple tree canker, obviously improve the prevention and treatment effect, obviously improve the activity of apple tree defensive enzyme, obviously increase the expression level of disease course related protein genes PR5 and PR8, obviously improve the content of free proline, obviously reduce the degree of membrane peroxidation, obviously enhance the resistance of apple trees to canker, reduce the incidence of apple tree canker by more than 95 percent, and achieve the cure rate of about 97.5 percent.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (6)
1. A Chinese herbal ointment for preventing and treating fruit tree rot is characterized in that: is prepared from folium Perillae ethanol extract 0.8g/mL, herba Violae 95% ethanol extract, ramulus Cinnamomi 95% ethanol extract, radix Sophorae Flavescentis 95% ethanol extract, radix Tripterygii Wilfordii 95% ethanol extract, surfactant sodium dodecyl sulfate, indoleacetic acid hot melt adhesive matrix and Borneolum Syntheticum.
2. The Chinese herbal ointment for preventing and treating rot of fruit trees as claimed in claim 1, which is characterized in that: the dosage of each raw material is as follows according to the mass ratio:
0.8g/mL of perilla leaf ethanol extract 1-9, 1-2.5 of corydalis 95% ethanol extract, 1-3 of cassia twig 95% ethanol extract, 1-3 of radix sophorae flavescentis 95% ethanol extract, 1-2.5 of tripterygium wilfordii 95% ethanol extract, 2-3 of surfactant lauryl sodium sulfate, 0.5-1 of borneol, 4-10 ppm of indoleacetic acid in total mass, and 20-7 of proportion of hot melt adhesive matrix to medicament.
3. The Chinese herbal ointment for preventing and treating rot of fruit trees as claimed in any one of claims 1 to 2, wherein: the herba Violae 95% ethanol extract, ramulus Cinnamomi 95% ethanol extract, radix Sophorae Flavescentis 95% ethanol extract, and radix Tripterygii Wilfordii 95% ethanol extract are respectively 100g herba Violae powder, ramulus Cinnamomi powder, radix Sophorae Flavescentis powder, and radix Tripterygii Wilfordii powder, respectively adding 1000ml ethanol solution with volume concentration of 95%, extracting at 40 deg.C for 2 hr for 3 times, and mixing the extractive solutions.
4. The preparation method of the Chinese herbal ointment for preventing and treating the rot disease of fruit trees as claimed in claim 1, which is characterized in that: the method comprises the following steps:
taking 1-9 parts of 0.8g/mL perilla leaf ethanol extract, 1-2.5 parts of corydalis bungeana 95% ethanol extract, 1-3 parts of cassia twig 95% ethanol extract, 1-3 parts of radix sophorae flavescentis 95% ethanol extract and 1-2.5 parts of tripterygium wilfordii 95% ethanol extract according to the mass ratio, sieving with an 80-mesh sieve, mixing, adding 2-3 parts of surfactant, stirring uniformly, adding 4-10 parts per million of indoleacetic acid, and stirring uniformly to obtain an agent A for later use;
and (3) putting the hot melt adhesive into a melter to be melted or heating the hot melt adhesive to 130 ℃ by using an iron pan to be completely melted, then turning to slow fire, pouring the agent A, uniformly stirring, adding 0.5-1 part of borneol, spreading the ointment immediately after stirring, putting the plaster on kraft paper, and putting the plaster on the surface of the plaster with oil covered by silicone oil paper.
5. The application of the Chinese herbal ointment for preventing and treating the rot disease of fruit trees as claimed in claim 1, which is characterized in that: can be used for preventing and treating rot of fruit trees.
6. The use of claim 4, wherein: the method comprises the following steps:
treating the lesion spots: and (3) cutting the disease spots into a cutting path of 0.8-1 cm by a knife, wherein the cutting path is along the growth direction of the trunk or the branches, the depth of the cutting groove reaches xylem, the range of the nick exceeds the range of the disease spots by 1-2 cm, the plaster is stuck to the treated disease spots and fixed, and the plaster is replaced once every 5-7 days, so that a good prevention and treatment effect can be achieved for three times.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111430879.XA CN113973851A (en) | 2021-11-29 | 2021-11-29 | Chinese herbal medicine ointment for preventing and treating fruit tree rot disease and preparation method and application thereof |
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| CN202111430879.XA CN113973851A (en) | 2021-11-29 | 2021-11-29 | Chinese herbal medicine ointment for preventing and treating fruit tree rot disease and preparation method and application thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1040126A (en) * | 1988-08-06 | 1990-03-07 | 徐立 | A kind of bactericide and using method that prevents apple canker |
| CN1413469A (en) * | 2002-06-14 | 2003-04-30 | 山西省农业科学院农产品综合利用研究所 | Chinese herbal medicine ointment preparation for preventing and curing rot of fruit tree |
| CN1593158A (en) * | 2004-07-12 | 2005-03-16 | 廉云鹏 | Fruit tree bactericide and its preparation method |
| CN106259500A (en) * | 2016-08-19 | 2017-01-04 | 滁州新源农业科技有限公司 | A kind of medicament preventing and treating apple tree canker and preparation method thereof |
| CN109769876A (en) * | 2019-03-29 | 2019-05-21 | 沈阳农业大学 | A kind of compound biological pesticide for preventing and controlling apple tree rot disease and preparation method thereof |
-
2021
- 2021-11-29 CN CN202111430879.XA patent/CN113973851A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1040126A (en) * | 1988-08-06 | 1990-03-07 | 徐立 | A kind of bactericide and using method that prevents apple canker |
| CN1413469A (en) * | 2002-06-14 | 2003-04-30 | 山西省农业科学院农产品综合利用研究所 | Chinese herbal medicine ointment preparation for preventing and curing rot of fruit tree |
| CN1593158A (en) * | 2004-07-12 | 2005-03-16 | 廉云鹏 | Fruit tree bactericide and its preparation method |
| CN106259500A (en) * | 2016-08-19 | 2017-01-04 | 滁州新源农业科技有限公司 | A kind of medicament preventing and treating apple tree canker and preparation method thereof |
| CN109769876A (en) * | 2019-03-29 | 2019-05-21 | 沈阳农业大学 | A kind of compound biological pesticide for preventing and controlling apple tree rot disease and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘芳洁: "核桃树腐烂病防治制剂配制及使用方法", 《农业工程》 * |
| 刘芳洁: "紫苏不同部位乙醇提取物对苹果树腐烂病的影响及机制", 《江苏农业科学》 * |
| 杨洪强: "《有机园艺》", 31 July 2005, 20050731 * |
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Application publication date: 20220128 |