SG10201914007YA - Non-static suspension culture of cell aggregates - Google Patents

Non-static suspension culture of cell aggregates

Info

Publication number
SG10201914007YA
SG10201914007YA SG10201914007YA SG10201914007YA SG10201914007YA SG 10201914007Y A SG10201914007Y A SG 10201914007YA SG 10201914007Y A SG10201914007Y A SG 10201914007YA SG 10201914007Y A SG10201914007Y A SG 10201914007YA SG 10201914007Y A SG10201914007Y A SG 10201914007YA
Authority
SG
Singapore
Prior art keywords
suspension culture
cell aggregates
static suspension
static
aggregates
Prior art date
Application number
SG10201914007YA
Inventor
Kartik Subramanian
Wei-Shou Hu
Catherine Verfaillie
Yonsil Park
Original Assignee
Univ Minnesota
Univ Leuven Kath
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota, Univ Leuven Kath filed Critical Univ Minnesota
Publication of SG10201914007YA publication Critical patent/SG10201914007YA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0692—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
SG10201914007YA 2010-08-24 2011-08-24 Non-static suspension culture of cell aggregates SG10201914007YA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37650810P 2010-08-24 2010-08-24

Publications (1)

Publication Number Publication Date
SG10201914007YA true SG10201914007YA (en) 2020-03-30

Family

ID=45697772

Family Applications (3)

Application Number Title Priority Date Filing Date
SG10201914007YA SG10201914007YA (en) 2010-08-24 2011-08-24 Non-static suspension culture of cell aggregates
SG2013013529A SG188280A1 (en) 2010-08-24 2011-08-24 Non-static suspension culture of cell aggregates
SG10201506664TA SG10201506664TA (en) 2010-08-24 2011-08-24 Non-static suspension culture of cell aggregates

Family Applications After (2)

Application Number Title Priority Date Filing Date
SG2013013529A SG188280A1 (en) 2010-08-24 2011-08-24 Non-static suspension culture of cell aggregates
SG10201506664TA SG10201506664TA (en) 2010-08-24 2011-08-24 Non-static suspension culture of cell aggregates

Country Status (8)

Country Link
US (2) US8609406B2 (en)
EP (1) EP2609191B1 (en)
CN (1) CN103237886B (en)
AU (1) AU2011293440B2 (en)
CA (1) CA2809245C (en)
IL (1) IL224775A (en)
SG (3) SG10201914007YA (en)
WO (1) WO2012027474A1 (en)

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SG10201914007YA (en) * 2010-08-24 2020-03-30 Univ Minnesota Non-static suspension culture of cell aggregates
WO2013123448A1 (en) * 2012-02-16 2013-08-22 Murry Charles Micro-tissue particles and methods for their use in cell therapy
WO2014026201A1 (en) * 2012-08-10 2014-02-13 The Trustees Of Columbia University In The City Of New York Injectable brown adipose microtissues for treatment and prevention of obesity and diabetes
WO2014138203A2 (en) * 2013-03-05 2014-09-12 Board Of Regents, The University Of Texas System Microfluidic devices for the rapid and automated processing of sample populations
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CA3069870A1 (en) * 2017-07-14 2019-01-17 The Governing Council Of The University Of Toronto Microfluidic platform for the rapid production of organoids/spheroids for compound screening
WO2019071106A1 (en) * 2017-10-06 2019-04-11 The Trustees Of The University Of Pennsylvania Tissue engineered "axon fusion" for immediate recovery following axon transection
CN108324993B (en) * 2018-01-15 2020-11-03 朱剑虹 Stem cell complex for inducing hair regeneration, preparation method and application thereof
SG11202106642YA (en) * 2018-12-20 2021-07-29 Sumitomo Chemical Co Embryonic erythroblast-containing cell population and method for producing same, cell culture composition, and compound test method
CN109628372A (en) * 2019-01-10 2019-04-16 武汉济源高科技有限公司 Culture of adherent cells by magnetic bead method
CN117757719A (en) * 2023-12-25 2024-03-26 中国人民解放军总医院第一医学中心 A microtissue that accelerates skin damage repair and its application

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Also Published As

Publication number Publication date
WO2012027474A1 (en) 2012-03-01
EP2609191B1 (en) 2017-11-22
CN103237886A (en) 2013-08-07
EP2609191A4 (en) 2014-04-30
US9447380B2 (en) 2016-09-20
AU2011293440A1 (en) 2013-03-14
CA2809245C (en) 2020-07-14
US20140024116A1 (en) 2014-01-23
AU2011293440B2 (en) 2016-05-05
EP2609191A1 (en) 2013-07-03
US20120052568A1 (en) 2012-03-01
IL224775A (en) 2017-11-30
AU2016206283A1 (en) 2016-08-11
SG188280A1 (en) 2013-04-30
SG10201506664TA (en) 2015-10-29
CN103237886B (en) 2018-10-30
US8609406B2 (en) 2013-12-17
CA2809245A1 (en) 2012-03-01

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