Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 May 11;356(3):594-8.
doi: 10.1016/j.bbrc.2007.03.026. Epub 2007 Mar 12.

Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit

Shu-Chen Kao  1 , Chun-Yu Chen, Shiu-Lan Wang, Jaw-Ji Yang, Wei-Chuan Hung, Yi-Chen Chen, Ning-Sheng Lai, Hsin-Tzu Liu, Hsien-lu Huang, Hong-Chen Chen, Ta-Hsien Lin, Hsien-bin Huang
Affiliations

Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit

Shu-Chen Kao et al. Biochem Biophys Res Commun. .

Abstract

We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin-Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

LinkOut - more resources