Enhanced healing of goat femur-defect using BMP7 gene-modified BMSCs and load-bearing tissue-engineered bone
- PMID: 19725097
- DOI: 10.1002/jor.20973
Enhanced healing of goat femur-defect using BMP7 gene-modified BMSCs and load-bearing tissue-engineered bone
Abstract
Segmental defect regeneration is still a clinical challenge. In this study, we investigated the feasibility of bone marrow stromal cells (BMSCs) infected with adenoviral vector containing the bone morphogenetic protein 7 gene (AdBMP7) and load-bearing to enhance bone regeneration in a critically sized femoral defect in the goat model. The defects were implanted with AdBMP7-infected BMSCs/coral (BMP7 group) or noninfected BMSCs/coral (control group), respectively, stabilized with an internal fixation rod and interlocking nails. Bridging of the segmental defects was evaluated by radiographs monthly, and confirmed by biomechanical tests. Much callus was found in the BMP7 group, and nails were taken off after 3 months of implantation, indicating that regenerated bone in the defect can be remodeled by load-bearing, whereas after 6 months in control group. After load-bearing, it is about 5 months; the mechanical property of newly formed bone in the BMP7 group was restored, but 8 months in control group. Our data suggested that the BMP7 gene-modified BMSCs and load-bearing can promote bone regeneration in segmental defects.
Similar articles
-
Tissue-engineered bone repair of goat-femur defects with osteogenically induced bone marrow stromal cells.Tissue Eng. 2006 Mar;12(3):423-33. doi: 10.1089/ten.2006.12.423. Tissue Eng. 2006. PMID: 16579676
-
[The feasibility of adenoviral co-transduction of BMP2 and BMP7 for the expression of recombinant human BMP2/7 heterodimer in rat bone marrow mesenchymal stem cells].Zhonghua Zheng Xing Wai Ke Za Zhi. 2016 Mar;32(2):125-29. Zhonghua Zheng Xing Wai Ke Za Zhi. 2016. PMID: 30024692 Chinese.
-
Repair of goat tibial defects with bone marrow stromal cells and beta-tricalcium phosphate.J Mater Sci Mater Med. 2008 Jun;19(6):2367-76. doi: 10.1007/s10856-007-3348-3. Epub 2007 Dec 25. J Mater Sci Mater Med. 2008. PMID: 18158615
-
Pre-Clinical Evaluation of Biological Bone Substitute Materials for Application in Highly Loaded Skeletal Sites.Biomolecules. 2020 Jun 9;10(6):883. doi: 10.3390/biom10060883. Biomolecules. 2020. PMID: 32526829 Free PMC article. Review.
-
A review of mouse critical size defect models in weight bearing bones.Bone. 2013 Jul;55(1):241-7. doi: 10.1016/j.bone.2013.02.002. Epub 2013 Feb 14. Bone. 2013. PMID: 23416847 Free PMC article. Review.
Cited by
-
Synergistic Effects of Vascular Endothelial Growth Factor on Bone Morphogenetic Proteins Induced Bone Formation In Vivo: Influencing Factors and Future Research Directions.Biomed Res Int. 2016;2016:2869572. doi: 10.1155/2016/2869572. Epub 2016 Dec 13. Biomed Res Int. 2016. PMID: 28070506 Free PMC article. Review.
-
Effect of rhBMP-2 and VEGF in a vascularized bone allotransplant experimental model based on surgical neoangiogenesis.J Orthop Res. 2013 Apr;31(4):561-6. doi: 10.1002/jor.22277. Epub 2012 Nov 28. J Orthop Res. 2013. PMID: 23192572 Free PMC article.
-
Musculoskeletal tissue engineering: Adipose derived stromal cell implementation for the treatment of osteoarthritis.Biomaterials. 2022 Jul;286:121544. doi: 10.1016/j.biomaterials.2022.121544. Epub 2022 May 6. Biomaterials. 2022. PMID: 35633592 Free PMC article. Review.
-
Tissue Engineering and Cell-Based Therapies for Fractures and Bone Defects.Front Bioeng Biotechnol. 2018 Jul 31;6:105. doi: 10.3389/fbioe.2018.00105. eCollection 2018. Front Bioeng Biotechnol. 2018. PMID: 30109228 Free PMC article. Review.
-
Long-term tracking of segmental bone healing mediated by genetically engineered adipose-derived stem cells: focuses on bone remodeling and potential side effects.Tissue Eng Part A. 2014 May;20(9-10):1392-402. doi: 10.1089/ten.TEA.2013.0314. Tissue Eng Part A. 2014. PMID: 24367947 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
