[Activin A and BMP-4 induce cardiomyocyte-like cells differentiation of human amniotic epithelial cells]
- PMID: 22295717
[Activin A and BMP-4 induce cardiomyocyte-like cells differentiation of human amniotic epithelial cells]
Abstract
Amniotic epithelial cells (AECs) have been expected to be a good cell source for stem cell-based cardiac repair. Activin A signaling is required for cardiac differentiation in human embryonic stem cells (ESCs), and bone morphogenetic protein-4 (BMP-4) is an important regulator that controls stem cell fates. Previous study has established an efficient protocol to generate cardiomyocytes from human ESCs via induction with Activin A and BMP-4. The aim of present study was to test the hypothesis that Activin A and BMP-4 could also induce AECs to differentiate into cardiomyocytes in vitro. Human AECs (hAECs) were isolated from human term placenta by trypsin digestion according to the previous reports. Freshly isolated hAECs were examined to detect the expression of cytokeratin 19 by immunocytochemistry. High-density undifferentiated hAECs at passage 1 were sequential treated with 100 ng/ ml human recombinant Actvin A and 10 ng/ml BMP-4. The expression of cardiac-specific genes was examined before and after in vitro induction of cellular differentiation. Freshly isolated hAEC could express cytokeratin 19, the specific marker of epithelial cells. The data showed that hAECs treated with Activin A and BMP-4 were able to express cardiac-specific genes, including Nkx2.5 and alpha-actinin. Our results demonstrated that Activin A and BMP-4 could induce cardiomyocyte differentiation of hAECs, which might be a novel approach to induce differentiation of AECs into cardiomyocytes-like cells.
Similar articles
-
Effect of bone morphogenetic protein-4 on cardiac differentiation from mouse embryonic stem cells in serum-free and low-serum media.Int J Cardiol. 2008 Jun 23;127(1):78-87. doi: 10.1016/j.ijcard.2007.04.173. Epub 2007 Aug 21. Int J Cardiol. 2008. PMID: 17714812
-
Effect of bone morphogenetic protein-4 (BMP-4) on cardiomyocyte differentiation from mouse embryonic stem cell.Int J Cardiol. 2007 Aug 9;120(1):92-101. doi: 10.1016/j.ijcard.2006.08.118. Epub 2006 Dec 6. Int J Cardiol. 2007. PMID: 17156864
-
Efficient cardiomyocyte differentiation of embryonic stem cells by bone morphogenetic protein-2 combined with visceral endoderm-like cells.Cell Biol Int. 2006 Oct;30(10):769-76. doi: 10.1016/j.cellbi.2006.05.011. Epub 2006 Jun 3. Cell Biol Int. 2006. PMID: 16831561
-
Production of hepatocyte-like cells from human amnion.Methods Mol Biol. 2009;481:155-68. doi: 10.1007/978-1-59745-201-4_13. Methods Mol Biol. 2009. PMID: 19096803 Review.
-
Stem cell characteristics and the therapeutic potential of amniotic epithelial cells.Am J Reprod Immunol. 2018 Oct;80(4):e13003. doi: 10.1111/aji.13003. Epub 2018 Jun 29. Am J Reprod Immunol. 2018. PMID: 29956869 Review.
Cited by
-
Application of human amniotic epithelial cells in regenerative medicine: a systematic review.Stem Cell Res Ther. 2020 Oct 15;11(1):439. doi: 10.1186/s13287-020-01951-w. Stem Cell Res Ther. 2020. PMID: 33059766 Free PMC article. Review.
-
Mesenchymal stem cells and immune disorders: from basic science to clinical transition.Front Med. 2019 Apr;13(2):138-151. doi: 10.1007/s11684-018-0627-y. Epub 2018 Jul 30. Front Med. 2019. PMID: 30062557 Review.
-
Cardiomyocyte differentiation of mesenchymal stem cells from bone marrow: new regulators and its implications.Stem Cell Res Ther. 2018 Feb 26;9(1):44. doi: 10.1186/s13287-018-0773-9. Stem Cell Res Ther. 2018. PMID: 29482607 Free PMC article. Review.