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. 2012 Mar;102-531(3):213-219.
doi: 10.1016/j.pestbp.2012.01.003.

Possible connection between imidacloprid-induced changes in rice gene transcription profiles and susceptibility to the brown plant hopper Nilaparvatalugens Stål (Hemiptera: Delphacidae)

Yao Cheng  1 , Zhao-Peng Shi, Li-Ben Jiang, Lin-Quan Ge, Jin-Cai Wu, Gary C Jahn
Affiliations

Possible connection between imidacloprid-induced changes in rice gene transcription profiles and susceptibility to the brown plant hopper Nilaparvatalugens Stål (Hemiptera: Delphacidae)

Yao Cheng et al. Pestic Biochem Physiol. 2012 Mar.

Abstract

The chemical pesticide, imidacloprid (IMI) has long-lasting effectiveness against Hemiptera. IMI is commonly used to control the brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae). Some chemical pesticides, however, can induce the susceptibility of rice to BPH, which has indirectly led to the resurgence of BPH. The mechanism of the chemical induction of the susceptibility of rice to BPH was not previously understood. Here, a 44 K Agilent Rice Expression Microarray was used to identify changes in gene expression that accompany IMI-induced rice susceptibility to BPH. The results showed that 225 genes were differentially expressed, of which 117 were upregulated, and 108 were downregulated. Gene ontology annotation and pathway analysis revealed that differentially expressed genes were mainly classified into the eight functional groups: oxidation reduction, regulation of cellular process, response to stress, electron carrier activity, metabolic process, transport, signal transducer, and organismal development. The genes encoding plant lipid transfer protein, lignin peroxidase, and flavonol-3-O-methyltransferenase may be important responses to the IMI-induced susceptibility of rice to BPH. The reliability of the microarray data was verified by performing quantitative real-time PCR and the data provide valuable information for further study of the molecular mechanism of IMI-induced susceptibility of rice.

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Figures

None
Graphical abstract
Fig. 1
Fig. 1
Effect of IMI on the injury level of rice plants infested by BPH. Numbers on the top correspond to the damage index under different IMI concentrations. Data are presented as the means ± SD. Means followed by different letters indicate significant differences at the 5% level.
Fig. 2
Fig. 2
Effects of IMI on the physiology and biochemistry of rice plants. (A) MDA content, (B) Oxalic acid content, (C) Photosynthetic rates, (D) Relative content of chlorophyll. Data are presented as the means ± SD. The different letters above the bars indicate that the means of these values are significantly different at a 5 % level.
Fig. 3
Fig. 3
Confirmation of the microarray results by qRT-PCR. Analysis by qRT-PCR of 10 genes selected from the IMI-responsive genes was performed with RNA extracted from control rice sheaths or 60 ppm IMI treated rice sheaths. The fold change of related genes after 60 ppm IMI treatment is presented on the left. The correlation between microarray signal ratios and qRT-PCR is presented on the right..
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