Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Nov 29;491(7426):711-6.
doi: 10.1038/nature11543. Epub 2012 Oct 17.

A physical, genetic and functional sequence assembly of the barley genome

International Barley Genome Sequencing Consortium; Klaus F X Mayer, Robbie Waugh, John W S Brown, Alan Schulman, Peter Langridge, Matthias Platzer, Geoffrey B Fincher, Gary J Muehlbauer, Kazuhiro Sato, Timothy J Close, Roger P Wise, Nils Stein
Collaborators
Free article

A physical, genetic and functional sequence assembly of the barley genome

International Barley Genome Sequencing Consortium et al. Nature. .
Free article

Abstract

Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.

PubMed Disclaimer

Comment in

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nat Biotechnol. 2010 May;28(5):511-5 - PubMed
    1. Science. 2009 Nov 20;326(5956):1112-5 - PubMed
    1. Nature. 2009 Jan 29;457(7229):551-6 - PubMed
    1. Nat Protoc. 2012 Mar 01;7(3):562-78 - PubMed
    1. Nat Genet. 2009 Feb;41(2):258-63 - PubMed

Publication types

MeSH terms

Substances