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. 2015 Jan;9(1):430-436.
doi: 10.3892/ol.2014.2682. Epub 2014 Nov 7.

Expression characteristics of FHIT, p53, BRCA2 and MLH1 in families with a history of oesophageal cancer in a region with a high incidence of oesophageal cancer

Zhiwei Chang  1 , Weijie Zhang  1 , Zhijun Chang  2 , Min Song  1 , Yanru Qin  1 , Fubao Chang  2 , Haiyun Guo  2 , Qingli Wei  2
Affiliations

Expression characteristics of FHIT, p53, BRCA2 and MLH1 in families with a history of oesophageal cancer in a region with a high incidence of oesophageal cancer

Zhiwei Chang et al. Oncol Lett. 2015 Jan.

Abstract

The aim of the present study was to determine the changes to the expression levels of fragile histidine triad (FHIT), breast cancer type 2 susceptibility protein (BRCA2), MutL homolog 1 (MLH1) and tumour protein 53 (p53) exhibited by families with a history of oesophageal cancer in a region that has a high incidence of oesophageal cancer, and to determine the association of these changes with the cancer history of the families. Immunohistochemistry was used to detect the protein expression of FHIT, p53, BRCA2, and MLH1 in the excised specimens of cancer tissues from 74 oesophageal cancer patients (positive family history of oesophageal cancer [OCFH +], n=33; negative family history of oesophageal cancer [OCFH -], n=41) from a region with a high incidence of oesophageal cancer. The positive expression rates of FHIT (61%; 45/74), BRCA2 (50%; 37/74) and MLH1 (27%; 9/33) in the oesophageal cancer tissues were significantly lower than those in the healthy tissues adjacent to the cancer (97% [29/30], 87% [26/30] and 73% [25/41], respectively). A significant difference was identified between the positive expression rates (P<0.01). However, FHIT, p53, BRCA2 and MLH1 expression demonstrated no significant affect on clinicopathological changes, such as oesophageal cancerous tissue differentiation, the degree of infiltration and cancer cell metastasis. The FHIT, BRCA2 and MLH1 expression levels were identified to be significantly lower in the cancer tissues from OCFH + patients. This result indicates that the expression levels of FHIT, BRCA2, and MLH1 are important molecular indices of genetic susceptibility to oesophageal cancer.

Keywords: breast cancer type 2 susceptibility protein; esophagus cancer; fragile histidine triad; genetic predisposition; mixed lymphocytic-histiocytic lymphoma; positive family history.

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Figures

Figure 1
Figure 1
Fragile histidine triad (FHIT) immune response in oesophageal carcinoma tissues. (A) Strong and positive reaction for FHIT expression. Immunoreactivity was predominantly located in the cytoplasm and nucleus; a positive cancer cell is shown by the arrow. (B) Weak and positive reaction for FHIT expression. Immunoreactivity was predominantly located in the cytoplasm and nucleus; a positive cancer cell is demonstrated by the arrow. (C) Negative reaction for FHIT expression; a negatively expressed cell is shown by the arrow. Magnification, ×400; haematoxylin and eosin restained.
Figure 2
Figure 2
Breast cancer type 2 susceptibility protein (BRCA2) immune response in oesophageal carcinoma tissues. (A) Strong and positive reaction for BRCA2 expression. Immunoreactivity was predominantly located in the cytoplasm and cell membrane; a positive cancer cell is shown by the arrow. (B) Weak and positive reaction for BRCA2 expression. Immunoreactivity was predominantly located in the cytoplasm and membrane; a positive cancer cell is shown by the arrow. (C) Negative expression for BRCA2; a negatively expressed cell is shown by the arrow. Magnification, ×400; haematoxylin and eosin restained.
Figure 3
Figure 3
Mixed lymphocytic histiocytic lymphoma (MLH1) immune response in oesophageal carcinoma tissues. (A) Positive reaction for MLH1 expression. Immunoreactivity was predominantly located in the nucleus; a positive cancer cell is shown by the arrow. (B) Negative reaction for MLH1 expression; a negatively expressed cell is shown by the arrow. Magnification, ×400; haematoxylin and eosin restained.
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References

    1. Du BL. Esophageal Carcinoma. Beijing: China Science and Technology Press; 1994. pp. 30–60.
    1. Wang LD, Gao WJ, Yang WC, et al. Prelimary analysis of the statistics on 3933 cases with esophageal cancer and gastric cardia cancer from the subjects in the People’s Hospital of Linzhou in 9 years. J Henan Med Univ. 1997;32:9–11. (In Chinese)
    1. Lu JB, Yang WX, Zu SK, et al. Cancer mortality and mortality trends in Henan, China, 1974–1985. Cancer Detect Prev. 1988;13:167–173. - PubMed
    1. Shi QL, Xu DZ, Sun C. Study on family aggregation of esophageal cancer in Linzhou city. Zhonghua Yu Fang Yi Xue Za Zhi. 2000;34:269–270. (In Chinese) - PubMed
    1. Hu N, Dawsey SM, Wu M, et al. Familial aggregation of oesophageal cancer in Yangcheng County, Shanxi Province, China. Int J Epidemiol. 1992;21:877–882. doi: 10.1093/ije/21.5.877. - DOI - PubMed

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