Polyploid tissues in the nematode Caenorhabditis elegans
- PMID: 2578115
- DOI: 10.1016/0012-1606(85)90381-1
Polyploid tissues in the nematode Caenorhabditis elegans
Abstract
During larval development, the number of somatic nuclei in C. elegans hermaphrodites increases from 558 to 959 (J. E. Sulston and H. R. Horvitz, Dev. Biol. 56, 110-156, 1977; J. E. Sulston et al., Dev. Biol. 100, 64-119, 1983). At the same time, the animals increase about 60-fold in volume. We have measured the DNA contents of several classes of nuclei by quantitating the fluorescence of Hoescht 33258 stained DNA (D. G. Albertson et al., Dev. Biol. 63, 165-178, 1978). Probably all embryonic nuclei, including those of neurons, muscles, hypodermis, and intestine, are diploid at hatching. Neurons, muscles, and nondividing hypodermal nuclei remain diploid throughout larval development. The DNA content of the intestinal nuclei doubles at the end of each larval stage, reaching 32C by the adult stage. New hypodermal cells, generated by division of seam cells in the larval stages, undergo an additional round of DNA replication before fusing with the major syncytium (hyp7, Sulston et al., 1983). Thus the larval hyp7 syncytium comprises a fixed number of diploid embryonic nuclei plus an increasing number of tetraploid postembryonic nuclei. Some of the endoreduplications that occur in the intestinal and hypodermal lineages of C. elegans may correspond to nuclear or cellular divisions in another nematode Panagrellus redivivus (P. W. Sternberg and H. R. Horvitz, Dev. Biol. 93, 181-205, 1982).
Similar articles
-
Coordinates, DNA content and heterogeneity of cell nuclei and segments of the Caenorhabditis elegans intestine.Histochem Cell Biol. 2005 Nov;124(5):359-67. doi: 10.1007/s00418-005-0039-3. Epub 2005 Nov 3. Histochem Cell Biol. 2005. PMID: 16158289
-
Loss of intestinal nuclei and intestinal integrity in aging C. elegans.Aging Cell. 2011 Aug;10(4):699-710. doi: 10.1111/j.1474-9726.2011.00713.x. Epub 2011 May 25. Aging Cell. 2011. PMID: 21501374 Free PMC article.
-
Cell fusions in the developing epithelial of C. elegans.Dev Biol. 1994 Feb;161(2):408-24. doi: 10.1006/dbio.1994.1041. Dev Biol. 1994. PMID: 8313992
-
Targeting cellular memory to reprogram the epigenome, restore potential, and improve somatic cell nuclear transfer.Anim Reprod Sci. 2007 Mar;98(1-2):129-46. doi: 10.1016/j.anireprosci.2006.10.019. Epub 2006 Oct 21. Anim Reprod Sci. 2007. PMID: 17166676 Review.
-
C. elegans cell cycles: invariance and stem cell divisions.Nat Rev Mol Cell Biol. 2005 Oct;6(10):766-76. doi: 10.1038/nrm1738. Nat Rev Mol Cell Biol. 2005. PMID: 16314866 Review.
Cited by
-
A novel mutation in β integrin reveals an integrin-mediated interaction between the extracellular matrix and cki-1/p27KIP1.PLoS One. 2012;7(8):e42425. doi: 10.1371/journal.pone.0042425. Epub 2012 Aug 6. PLoS One. 2012. PMID: 22879977 Free PMC article.
-
Coordinates, DNA content and heterogeneity of cell nuclei and segments of the Caenorhabditis elegans intestine.Histochem Cell Biol. 2005 Nov;124(5):359-67. doi: 10.1007/s00418-005-0039-3. Epub 2005 Nov 3. Histochem Cell Biol. 2005. PMID: 16158289
-
Endoreplication: polyploidy with purpose.Genes Dev. 2009 Nov 1;23(21):2461-77. doi: 10.1101/gad.1829209. Genes Dev. 2009. PMID: 19884253 Free PMC article. Review.
-
Nuclear lipid droplets and nuclear damage in Caenorhabditis elegans.PLoS Genet. 2021 Jun 16;17(6):e1009602. doi: 10.1371/journal.pgen.1009602. eCollection 2021 Jun. PLoS Genet. 2021. PMID: 34133414 Free PMC article.
-
Chromosomal clustering and GATA transcriptional regulation of intestine-expressed genes in C. elegans.Development. 2006 Jan;133(2):287-95. doi: 10.1242/dev.02185. Epub 2005 Dec 14. Development. 2006. PMID: 16354718 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
