Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 Jul 1;136(1):143-55.
doi: 10.1084/jem.136.1.143.

Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator(s)

I Gery, B H Waksman

Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator(s)

I Gery et al. J Exp Med. .

Abstract

Effective supernatants (SUP), which potentiate mouse T-cell responses to phytohemagglutin (PHA), are obtained from cells of several species (human, rabbit, rat, mouse) and indeed from syngeneic spleen, thymus, or bone marrow cells. Unstimulated cells release some SUP activity but more is produced after stimulation. Lipopolysaccharide (LPS) produced very active SUP in all cultures tested. PHA was similarly active on human leukocytes only, whereas concanavalin A (Con A) gave highly efficient SUP only with mouse spleen cells. SUP production is not correlated with a mitotic response of the donor cells and is observed in cultures unable to respond mitotically to the stimulant. Adherent mouse spleen cell populations, consisting largely or entirely of macrophages, produce active SUP, while nonadherent cells do not. Similarly, purification of human peripheral leukocytes on nylon columns, with removal of macrophages and other adherent cells, destroys their ability to produce SUP. The importance of indirect effects in stimulating mitotic responses of T cells is emphasized by the fact that the mitotic response of mouse thymocytes to LPS and its ability to potentiate the response of these cells to PHA disappears with removal of adherent cells from the thymocyte population. Conversely the production of SUP from spleen cells stimulated by Con A requires the presence of T cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Allergy. 1969 Jun;43(6):323-35 - PubMed
    1. Prog Allergy. 1970;14:81-144 - PubMed
    1. J Immunol. 1972 Apr;108(4):1088-91 - PubMed
    1. J Exp Med. 1971 Mar 1;133(3):520-33 - PubMed
    1. Cell Immunol. 1971 Aug;2(4):335-40 - PubMed