Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Nov 11;22(22):4574-82.
doi: 10.1093/nar/22.22.4574.

Molecular cloning and characterization of a human PAX-7 cDNA expressed in normal and neoplastic myocytes

B W Schäfer  1 , T Czerny, M Bernasconi, M Genini, M Busslinger
Affiliations
Free PMC article

Molecular cloning and characterization of a human PAX-7 cDNA expressed in normal and neoplastic myocytes

B W Schäfer et al. Nucleic Acids Res. .
Free PMC article

Abstract

The myogenic basic helix-loop-helix proteins are essential components of the regulatory network controlling vertebrate myogenesis. However, determined myoblasts appear in the limb buds which do not initially express any member of this transcription factor family. In a search for potential novel regulators of myogenesis, a human PAX-7 cDNA was isolated from primary myoblasts. Analysis of the DNA-binding properties of the Pax-7 paired domain revealed that it binds DNA in a sequence-specific manner indistinguishable from that of the paralogous Pax-3 protein. Each of the two proteins also binds to palindromic homeodomain-binding sites by cooperative dimerization. Both Pax-3 and Pax-7, which are known to partially overlap in their expression during development, can also efficiently form heterodimers on these sites and stimulate reporter gene transcription in transient transfection experiments which, in the case of Pax-7, is dependent on the transactivation function encoded by the C-terminal sequences. Thus, the formation of heterodimers might have important consequences for target gene recognition and regulation during development. PAX-7 was found to be weakly expressed in normal human myoblasts, while PAX-3 could not be detected in these cells at all. However, transcripts for either PAX-3 and/or PAX-7 were expressed at elevated levels in tumorigenic rhabdomyosarcoma cell lines. Hence, overexpression of these PAX genes may be involved in the genesis of myogenic tumors.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
    1. Development. 1994 Apr;120(4):957-71 - PubMed
    1. Cell. 1988 Nov 4;55(3):531-5 - PubMed
    1. EMBO J. 1989 Apr;8(4):1183-90 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Jul;86(14):5434-8 - PubMed

Publication types

MeSH terms

Associated data