Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins
- PMID: 8001118
- DOI: 10.1016/0092-8674(94)90070-1
Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins
Abstract
We have used the interaction trap, a yeast two-hybrid system, to identify proteins interacting with hairy, a basic-helix-loop-helix (bHLH) protein that represses transcription during Drosophila embryonic segmentation. We find that the groucho (gro) protein binds specifically to hairy and also to hairy-related bHLH proteins encoded by deadpan and the Enhancer of split complex. The C-terminal WRPW motif present in all these bHLH proteins is essential for this interaction. We demonstrate that these associations reflect in vivo maternal requirements for gro during neurogenesis, segmentation, and sex determination, three processes regulated by the above bHLH proteins, and we propose that gro is a transcriptional corepressor recruited to specific target promoters by hairy-related bHLH proteins.
Similar articles
-
The WRPW motif of the hairy-related basic helix-loop-helix repressor proteins acts as a 4-amino-acid transcription repression and protein-protein interaction domain.Mol Cell Biol. 1996 Jun;16(6):2670-7. doi: 10.1128/MCB.16.6.2670. Mol Cell Biol. 1996. PMID: 8649374 Free PMC article.
-
Specificity for the hairy/enhancer of split basic helix-loop-helix (bHLH) proteins maps outside the bHLH domain and suggests two separable modes of transcriptional repression.Mol Cell Biol. 1995 Dec;15(12):6923-31. doi: 10.1128/MCB.15.12.6923. Mol Cell Biol. 1995. PMID: 8524259 Free PMC article.
-
Identification and expression of a novel family of bHLH cDNAs related to Drosophila hairy and enhancer of split.Biochem Biophys Res Commun. 1999 Jul 5;260(2):459-65. doi: 10.1006/bbrc.1999.0880. Biochem Biophys Res Commun. 1999. PMID: 10403790
-
Vertebrate hairy and Enhancer of split related proteins: transcriptional repressors regulating cellular differentiation and embryonic patterning.Oncogene. 2001 Dec 20;20(58):8342-57. doi: 10.1038/sj.onc.1205094. Oncogene. 2001. PMID: 11840327 Review.
-
Expression and function of Enhancer of split bHLH proteins during Drosophila neurogenesis.Perspect Dev Neurobiol. 1997;4(4):313-23. Perspect Dev Neurobiol. 1997. PMID: 9171445 Review.
Cited by
-
Groucho co-repressor proteins regulate β cell development and proliferation by repressing Foxa1 in the developing mouse pancreas.Development. 2021 Mar 24;148(6):dev192401. doi: 10.1242/dev.192401. Development. 2021. PMID: 33658226 Free PMC article.
-
Essential roles of Da transactivation domains in neurogenesis and in E(spl)-mediated repression.Mol Cell Biol. 2012 Nov;32(22):4534-48. doi: 10.1128/MCB.00827-12. Epub 2012 Sep 4. Mol Cell Biol. 2012. PMID: 22949507 Free PMC article.
-
Delta-Notch--and then? Protein interactions and proposed modes of repression by Hes and Hey bHLH factors.Nucleic Acids Res. 2007;35(14):4583-96. doi: 10.1093/nar/gkm477. Epub 2007 Jun 22. Nucleic Acids Res. 2007. PMID: 17586813 Free PMC article. Review.
-
Systematic discovery of new recognition peptides mediating protein interaction networks.PLoS Biol. 2005 Dec;3(12):e405. doi: 10.1371/journal.pbio.0030405. Epub 2005 Nov 15. PLoS Biol. 2005. PMID: 16279839 Free PMC article.
-
Capicua integrates input from two maternal systems in Drosophila terminal patterning.EMBO J. 2004 Nov 24;23(23):4571-82. doi: 10.1038/sj.emboj.7600457. Epub 2004 Oct 28. EMBO J. 2004. PMID: 15510215 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
