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Comparative Study
. 1994 Sep 13;91(19):8797-801.
doi: 10.1073/pnas.91.19.8797.

Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53

M Scheffner  1 , J M Huibregtse, P M Howley
Affiliations
Comparative Study

Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53

M Scheffner et al. Proc Natl Acad Sci U S A. .

Abstract

The E6 protein of the oncogenic human papillomavirus types 16 and 18 facilitates the rapid degradation of the tumor-suppressor protein p53 via the ubiquitin-dependent proteolytic pathway. The E6 protein binds to a cellular protein of 100 kDa termed E6-AP. The complex of E6 and E6-AP specifically interacts with p53 and induces the ubiquitination of p53 in a reaction which requires the ubiquitin-activating enzyme (E1) and a cellular fraction thought to contain a mammalian ubiquitin-conjugating enzyme (E2). This mammalian E2 activity could be replaced with bacterially expressed UBC8 from Arabidopsis thaliana, which belongs to a subfamily of E2s including yeast UBC4 and UBC5 which are highly conserved at the amino acid level. In this paper we describe the cloning of a human cDNA encoding a human E2 that we have designated UbcH5 and that is related to Arabidopsis UBC8 and the other members of this subfamily. We demonstrate that UbcH5 can function in the E6/E6-AP-induced ubiquitination of p53.

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References

    1. Cell. 1993 Nov 5;75(3):495-505 - PubMed
    1. Plant J. 1993 Apr;3(4):545-52 - PubMed
    1. Mol Cell Biol. 1994 Mar;14(3):1997-2003 - PubMed
    1. J Biol Chem. 1994 Apr 1;269(13):9574-81 - PubMed
    1. J Biol Chem. 1994 Apr 1;269(13):9582-9 - PubMed

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