The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro
Abstract
Cell-free extracts prepared from human 293 cells, supplemented with purified SV40 large-T antigen, support replication of plasmids containing the SV40 origin of DNA replication. A cellular protein (Mr ∼36,000) that is required for efficient SV40 DNA synthesis in vitro has been purified from these extracts. This protein is recognized by human autoantibodies and is identified as the cell-cycle regulated protein known as proliferating cell nuclear antigen (PCNA) or cyclin.
- Publication:
-
Nature
- Pub Date:
- April 1987
- DOI:
- Bibcode:
- 1987Natur.326..471P